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Product Name:

Rat Baculoviral IAP repeat-containing protein 4(XIAP) ELISA kit

Product Type : ELISA Kit
Code : CSB-EL026193RA
Size : 96T,5×96T,10×96T
Uniprot NO. : Q9R0I6
Abbreviation : XIAP
Protein Biological Process 1 : Apoptosis/Autophagy
Alias : RP1-315G1.5, API3, BIRC4, ILP1, MIHA, XLP2, OTTHUMP00000196392|apoptosis inhibitor 3|baculoviral IAP repeat-containing 4|baculoviral IAP repeat-containing protein 4
Species : Rat
Protein Biological Process 3 : Apoptosis
Sample Types : serum, plasma, tissue homogenates, cell lysates
Detect Range : 23.5 pg/ml-1500 pg/ml
Sensitivity : 5.88 pg/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 5-7 working days
Research Area : Cancer
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : This protein is a member of a family of proteins which inhibit apoptosis through binding to tumor necrosis factor receptor-associated factors TRAF1 and TRAF2. This protein inhibits apoptosis induced by menadione, a potent inducer of free radicals, and ICE. It also inhibits at least two members of the caspase family of cell-death proteases, caspase-3 and caspase-7.
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of rat XIAP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 86-93
1:2 Average % 98
Range % 92-104
1:4 Average % 88
Range % 86-93
1:8 Average % 89
Range % 84-96
Recovery :
The recovery of rat XIAP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 88-97
EDTA plasma (n=4) 100 95-102
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.932 2.933 2.933 2.806
750 1.944 2.110 2.027 1.900
375 1.335 1.445 1.390 1.263
187.5 0.833 0.845 0.839 0.712
94 0.541 0.566 0.554 0.427
47 0.332 0.340 0.336 0.209
23.5 0.223 0.230 0.227 0.100
0 0.131 0.123 0.127  
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