Rat Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP ELISA Kit

Code CSB-E08081r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Carboxyterminal propeptide of type Ⅰ procollagen,PⅠCP
Alternative Names
Carboxyterminal propeptide of type 1 procollagen,P1CP,Carboxyterminal propeptide of type I procollagen,PICP
Abbreviation
PⅠCP
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma
Detection Range
3.12 ng/mL-200 ng/mL
Sensitivity
0.78 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PⅠCP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 87  
Range % 82-96  
1:2 Average % 97  
Range % 91-103  
1:4 Average % 92  
Range % 88-97  
1:8 Average % 95  
Range % 91-99  
Recovery
The recovery of rat PⅠCP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 84 80-89  
EDTA plasma (n=4) 90 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
200 2.191 2.205 2.198 2.026  
100 1.578 1.611 1.595 1.423  
50 0.912 0.931 0.922 0.750  
25 0.603 0.625 0.614 0.442  
12.5 0.416 0.405 0.411 0.239  
6.25 0.319 0.297 0.308 0.136  
3.12 0.244 0.241 0.243 0.071  
0 0.173 0.170 0.172    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat PⅠCP ELISA Kit was designed for the quantitative measurement of Rat PⅠCP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL .

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