Rat Collagen Type Ⅱ,Col Ⅱ ELISA Kit

Code CSB-E08092r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Collagen Type Ⅱ,Col Ⅱ
Alternative Names
Collagen Type 2 ,Col 2, Collagen Type II,Col II
Abbreviation
Col Ⅱ
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat Col Ⅱ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 87  
Range % 83-92  
1:200 Average % 94  
Range % 90-99  
1:400 Average % 92  
Range % 88-96  
1:800 Average % 102  
Range % 98-105  
Recovery
The recovery of rat Col Ⅱ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 93-96  
EDTA plasma (n=4) 98 89-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.584 2.599 2.592 2.438  
500 2.217 2.253 2.235 2.081  
250 1.765 1.792 1.779 1.625  
125 1.292 1.264 1.278 1.124  
62.5 0.789 0.773 0.781 0.627  
31.2 0.396 0.388 0.392 0.238  
15.6 0.257 0.269 0.263 0.109  
0 0.153 0.155 0.154    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat Col Ⅱ ELISA Kit was designed for the quantitative measurement of Rat Col Ⅱ protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .

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