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Product Name:

Rat Estradiol,E2 ELISA Kit

Product Type : ELISA Kit
Code : CSB-E05110r
Size : 96T,5×96T,10×96T
Abbreviation : E2
Protein Biological Process 1 : Sex hormone
Alias : N/A
Species : Rat
Sample Types : serum, plasma, cell culture supernates, tissue homogenates
Detect Range : 20 pg/ml-1600 pg/ml
Sensitivity : 25pg/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision :
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of rat E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %85-95
1:2Average %96
Range %82-102
1:4Average %94
Range %90-101
1:8Average %98
Range %85-100
Recovery :
The recovery of rat E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9690-104
EDTA plasma (n=4)9590-99
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
14000.312 0.316 0.314
7000.483 0.459 0.471
3500.654 0.673 0.664
1201.058 0.986 1.022
402.339 2.417 2.378
01.944 2.005 1.975
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