Rat Substance P,SP ELISA Kit

Code CSB-E08358r
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
Substance P,SP
Alternative Names
N/A
Abbreviation
SP
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
6.25 pg/mL-400 pg/mL
Sensitivity
1.56 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Neuroscience
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 91  
Range % 86-95  
1:2 Average % 102  
Range % 98-106  
1:4 Average % 92  
Range % 87-95  
1:8 Average % 97  
Range % 91-103  
Recovery
The recovery of rat SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 90-97  
EDTA plasma (n=4) 96 91-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 1.738 1.682 1.710 1.618  
200 1.376 1.444 1.410 1.318  
100 1.066 1.045 1.056 0.964  
50 0.672 0.666 0.669 0.577  
25 0.448 0.434 0.441 0.349  
12.5 0.302 0.296 0.299 0.207  
6.25 0.152 0.157 0.155 0.063  
0 0.093 0.091 0.092    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Substance P, SP ELISA Kit is a perfect tool for neuroscience researchers studying Rattus norvegicus. This kit is specifically designed to detect Substance P in a variety of sample types, including serum, plasma, cell culture supernates, and tissue homogenates. With a detection range of 6.25 pg/mL-400 pg/mL and a sensitivity of 1.56 pg/mL, you can be sure of accurate and reliable results.

The assay principle is quantitative and employs a sandwich measurement technique. The assay time is 1-5h, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, providing precise and dependable readings.

This Rat Substance P, SP ELISA Kit has been cited in over 6 research publications and is an excellent choice for neuroscience researchers.

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