Code | CSB-E08358r |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of rat SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 91 | ||||
Range % | 86-95 | |||||
1:2 | Average % | 102 | ||||
Range % | 98-106 | |||||
1:4 | Average % | 92 | ||||
Range % | 87-95 | |||||
1:8 | Average % | 97 | ||||
Range % | 91-103 |
The recovery of rat SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 94 | 90-97 | ||||
EDTA plasma (n=4) | 96 | 91-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
The Rat Substance P, SP ELISA Kit is a perfect tool for neuroscience researchers studying Rattus norvegicus. This kit is specifically designed to detect Substance P in a variety of sample types, including serum, plasma, cell culture supernates, and tissue homogenates. With a detection range of 6.25 pg/mL-400 pg/mL and a sensitivity of 1.56 pg/mL, you can be sure of accurate and reliable results.
The assay principle is quantitative and employs a sandwich measurement technique. The assay time is 1-5h, and the sample volume required is 50-100ul. The detection wavelength is 450 nm, providing precise and dependable readings.
This Rat Substance P, SP ELISA Kit has been cited in over 6 research publications and is an excellent choice for neuroscience researchers.
There are currently no reviews for this product.