Rat Sulfotransferase Family 1E Member 1(SULT1E1) ELISA Kit

Code CSB-E13472r
Size 96T,5×96T,10×96T
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Product Details

Target Name
sulfotransferase family 1E, estrogen-preferring, member 1
Alternative Names
Sult1e1 ELISA Kit; Ste ELISA Kit; Ste1 ELISA Kit; Sulfotransferase 1E1 ELISA Kit; ST1E1 ELISA Kit; EC 2.8.2.4 ELISA Kit; Estrogen sulfotransferase ELISA Kit; isoform 1 ELISA Kit; EST-1 ELISA Kit; Estrone sulfotransferase ELISA Kit; Sulfotransferase ELISA Kit; estrogen-preferring ELISA Kit
Abbreviation
SULT1E1
Uniprot No.
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Others
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat SULT1E1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %86-93
1:2Average %108
Range %99-111
1:4Average %92
Range %87-97
1:8Average %99
Range %92-103
Recovery
The recovery of rat SULT1E1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9487-97
EDTA plasma (n=4)9990-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.488 2.377 2.433 2.300
52.075 2.045 2.060 1.927
2.51.412 1.389 1.401 1.268
1.250.916 0.898 0.907 0.774
0.6250.574 0.564 0.569 0.436
0.3120.353 0.367 0.360 0.227
0.1560.267 0.260 0.264 0.131
00.134 0.132 0.133
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Rat SULT1E1 ELISA Kit was designed for the quantitative measurement of Rat SULT1E1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfate conjugation of estradiol and estrone. Is a key enzyme in estrogen homeostasis, the sulfation of estrogens leads to their inactivation. Also sulfates dehydroepiandrosterone (DHEA), pregnenolone, (24S)-hydroxycholesterol and xenobiotic compounds like ethinylestradiol, equalenin, diethyl stilbesterol and 1-naphthol at significantly lower efficiency. Does not sulfonate cortisol, testosterone and dopamine.
Subcellular Location
Cytoplasm, cytosol.
Protein Families
Sulfotransferase 1 family
Database Links

UniGene: Rn.32104

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