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- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-rat TSH antibody.
- Standard 7 x 0.5 ml--- Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
- HRP-conjugated TSH antibody 1 x 6 ml --- Bind to the TSH in the sample or in the standard and react with the TMB substrate to make the solution chromogenic.
- Wash Buffer (20 x concentrate) 1 x 15 ml --- Wash away unbound or free substances.
- Substrate A1 x 7 ml --- Mix with Substrate B, and the mixture reacts with HRP to make the solution appear blue.
- Substrate B 1 x 7 ml --- Mix with Substrate A, and the mixture reacts with HRP to make the solution appear blue.
- Stop Solution 1 x 7 ml --- Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
