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| Code | CSB-E04576r |
| Size | 96T,5×96T,10×96T |
| Price | Request a Quote or Start an on-line Chat |
| Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
The rat ICAM1 ELISA Kit allows for the in vitro quantitative determination of ICAM1 concentrations in serum, plasma, tissue homogenates, or cell lysates. This kit exclusively recognizes rat ICAM1. ICAM1 is an adhesion receptor renowned for its ability to recruit leukocytes from circulation to inflamed sites. ICAM1 is constitutively lowly expressed in immune, endothelial and epithelial cells, but its expression is significantly elevated in response to inflammatory stimuli, such as IL-1, IL-6, and TNFα. In addition to its functions in the adhesion of cells, transendothelial migration of leukocytes to sites of inflammation, and activation of lymphocytes, ICAM1 has different roles in epithelial injury-resolution responses, innate and adaptive immune responses inflammation, and tumorigenesis. Increased ICAM1 expression has been detected in several malignant tumors, including breast, gastric, and colorectal cancers.
The detection mechanism of this kit is based on the Sandwich-ELISA technique. ICAM1 in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled ICAM1 antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with ICAM1 content in the sample. The kit has been quality-controlled with high sensitivity, strong specificity, good linearity, high precision, high recovery, and high lot-to-lot consistency.
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