Register | Sign In Professional & Accurate! CUSABIO is your good partner in biology research.
cusabio

Product Name:

Rat matrix metalloproteinase 9/Gelatinase B,MMP-9 ELISA Kit

Product Type : ELISA Kit
Code : CSB-E08008r
Size : 96T,5×96T,10×96T
Uniprot NO. : P50282
Abbreviation : MMP9
Protein Biological Process 1 : Developmental Protein
Alias : CLG4B, GELB, MANDP2, MMP-9, macrophage gelatinase|matrix metalloproteinase 9|matrix metalloproteinase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)|type V collagenase
Species : Rat
Protein Biological Process 3 : Collagen degradation
Sample Types : serum, plasma, cell culture supernates, tissue homogenates
Detect Range : 15.6 pg/ml-1000 pg/ml
Sensitivity : 3.9pg/ml
Assay Time : 1-5h
Sample Volume : 50-100ul
Detection Wavelengt : 450 nm
Availability : 3-5 working days
Protocol :
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Target details : Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.
HGNC : 7176
RGD : 621320
MGI : 97011
Precision :
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity :
To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:100Average %90
Range %86-96
1:200Average %97
Range %92-102
1:400Average %85
Range %81-88
1:800Average %89
Range %83-94
Recovery :
The recovery of rat MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9187-94
EDTA plasma (n=4)9692-101
Typical Data :
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.618 2.774 2.696 2.553
5002.119 2.133 2.126 1.983
2501.507 1.635 1.571 1.428
1250.865 0.867 0.866 0.723
62.50.521 0.532 0.527 0.384
31.20.385 0.405 0.395 0.252
15.60.256 0.246 0.251 0.108
00.142 0.144 0.143
Contact us
Copyright © 2007-2015 www.cusabio.com Cusabio Biotech Co., Ltd All Rights Reserved.
skype
Top