Rat tissue factor(TF)/coagulation factor VII(F7) complex ELISA kit

Code CSB-EQ027252RA
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
tissue factor(TF)/coagulation factor VII(F7) complex
Abbreviation
TF
Species
Rattus norvegicus (Rat)
Sample Types
serum, plasma, tissue homogenates
Detection Range
15.6 pg/mL-1000 pg/mL
Sensitivity
3.9 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat TF/F7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 107
Range % 98-110
1:2 Average % 103
Range % 96-107
1:4 Average % 98
Range % 89-102
1:8 Average % 105
Range % 98-110
Recovery
The recovery of rat TF/F7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 91 85-96
EDTA plasma (n=4) 102 95-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1000 2.708 2.673 2.691 2.589
500 2.243 2.150 2.197 2.095
250 1.419 1.469 1.444 1.342
125 0.702 0.734 0.718 0.616
62.5 0.412 0.425 0.419 0.317
31.2 0.251 0.263 0.257 0.155
15.6 0.167 0.172 0.170 0.068
0 0.101 0.103 0.102  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

The Rat Tissue Factor (TF)/Coagulation Factor VII (F7) Complex ELISA Kit is the perfect solution for researchers studying the cardiovascular system. This quantitative assay kit allows for the detection of TF/F7 complex in serum, plasma, and tissue homogenates of Rattus norvegicus (rat) samples.

With a detection range of 15.6 pg/mL to 1000 pg/mL and a sensitivity of 3.9 pg/mL, this ELISA kit provides accurate and reliable results for your research needs. The assay time is quick, ranging from 1-5 hours, and the sample volume required is only 50-100 ul.

The detection wavelength for this kit is 450 nm, providing high sensitivity and specificity for the measurement of TF/F7 complex in rat samples. The assay principle is based on the sandwich method, providing high specificity and accuracy for the detection of this complex.

The Rat Tissue Factor (TF)/Coagulation Factor VII (F7) Complex ELISA Kit is ideal for researchers working in the cardiovascular field, and provides a powerful tool for the study of TF/F7 complex in rat samples.

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