Target Name
matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase)
Alternative Names
MMP9Matrix metalloproteinase-9 ELISA Kit; MMP-9 ELISA Kit; EC 3.4.24.35 ELISA Kit; 92 kDa gelatinase ELISA Kit; 92 kDa type IV collagenase ELISA Kit; Gelatinase B ELISA Kit; GELB ELISA Kit
Species
Oryctolagus cuniculus (Rabbit)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
23.5 pg/mL-1500 pg/mL
Detection Wavelength
450 nm
Assay Principle
quantitative
Precision
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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Linearity
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
99 |
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| Range % |
96-102 |
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| 1:40 |
Average % |
85 |
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| Range % |
92-99 |
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| 1:80 |
Average % |
101 |
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| Range % |
98-104 |
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| 1:160 |
Average % |
85 |
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| Range % |
82-89 |
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Recovery
| The recovery of rabbit MMP-9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
93-99 |
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| EDTA plasma (n=4) |
100 |
96-104 |
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Typical Data
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
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| 1500 |
0.129 |
0.127 |
0.128 |
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| 750 |
0.226 |
0.238 |
0.232 |
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| 375 |
0.379 |
0.370 |
0.375 |
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| 187.5 |
0.572 |
0.565 |
0.569 |
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| 94 |
0.799 |
0.785 |
0.792 |
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| 47 |
0.937 |
0.987 |
0.962 |
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| 23.5 |
1.148 |
1.168 |
1.158 |
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| 0 |
1.606 |
1.634 |
1.620 |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Rabbit MMP9 ELISA Kit was designed for the quantitative measurement of Rabbit MMP9 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Competitive ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.
