Human anti-cytomegalovirus(CMV) antibody ELISA Kit , IgM ELISA Kit Human Hepatitis B e Antigen ELISA Kit , HBeAg ELISA Kit Human anti-epidemic hemorrhagic fever virus antibody ELISA Kit , IgG ELISA Kit Human anti-sperm antibody ELISA Kit , AsAb ELISA Kit Rabbit arachidonic Acid ELISA Kit , AA ELISA Kit Dog Immunoglobulin G ELISA Kit , IgG ELISA Kit Pigeon Serum albumin ELISA Kit , ALB ELISA Kit Guinea pig Serum albumin ELISA Kit , ALB ELISA Kit Hamster apolipoprotein B ELISA Kit , APOB ELISA Kit Guinea pig Apolipoprotein B ELISA Kit , APOB ELISA Kit Bovine Apolipoprotein B ELISA Kit , APOB ELISA Kit Horse Apolipoprotein B ELISA Kit , APOB ELISA Kit Goat Apolipoprotein B ELISA Kit , APOB ELISA Kit Sheep Apolipoprotein B ELISA Kit , APOB ELISA Kit Dog Apolipoprotein B ELISA Kit , APOB ELISA Kit
DUSP6 cDNA Clone USP21 cDNA Clone PRELP cDNA Clone GJA1 cDNA Clone PPM1A cDNA Clone PRKAR2A cDNA Clone MAPKAPK3 cDNA Clone SDR42E1 cDNA Clone Recombinant Streptococcus equi subsp. zooepidemicus Glucose-6-phosphate isomerase(pgi) for Recombinant Streptococcus equi subsp. zooepidemicus Peptide chain release factor 1(prfA) for Recombinant Streptococcus equi subsp. zooepidemicus Peptide deformylase(def) for Recombinant Hydrogenobaculum sp. Elongation factor G(fusA) for Recombinant Vibrio cholerae serotype O1 2-dehydropantoate 2-reductase(panE) for Recombinant Vibrio cholerae serotype O1 Ribosomal protein S6 modification protein(rimK) for Recombinant Vibrio cholerae serotype O1 [Protein-PII] uridylyltransferase(glnD) for
At5g42460 Recombinant ProteinAt5g42700 Recombinant ProteinAt5g42850 Recombinant ProteinAt5g43190 Recombinant ProteinAt5g43285 Recombinant ProteinAt5g43401 Recombinant ProteinAt5g43440 Recombinant ProteinAt5g43450 Recombinant ProteinAt5g43510 Recombinant ProteinAt5g43513 Recombinant ProteinAt5g43516 Recombinant ProteinAt5g43530 Recombinant ProteinAt5g43560 Recombinant ProteinAt5g43730 Recombinant ProteinAt5g43740 Recombinant Protein
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- ELISA Kit
- Rat Caspase 3 , Casp-3 ELISA Kit
- This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer s disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq]
Rat Caspase 3 , Casp-3 ELISA Kit
||CPP32, CPP32B, SCA-1, PARP cleavage protease|SREBP cleavage activity 1|Yama|apopain|caspase 3|caspase 3, apoptosis-related cysteine protease|cysteine protease CPP32|procaspase3|
|other species:||Bovine Cat Dog Mouse Pig Rabbit Human|
||caspase 3, apoptosis-related cysteine peptidase|
|Protein Biological Process 1:
|Protein Biological Process 3:
||0.312 ng/ml-20 ng/ml|
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
|Specificty:||This assay has high sensitivity and excellent specificity for detection of CASP3. No significant cross-reactivity or interference between CASP3 and analogues was observed.|
|Precision:||Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
|Sample collection and storage:||Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 4°C before centrifugation for 15 minutes at 1000 ×g. Remove serum and assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 ×g at 2-8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at -20°C or -80°C. Avoid repeated freeze-thaw cycles.
|Assay procedure:||Bring all reagents and samples to room temperature before use. Centrifuge the sample again after thawing before the assay. It is recommended that all samples and standards be assayed in duplicate.
1. Prepare all reagents, working standards, and samples as directed in the previous sections.
2. Refer to the Assay Layout Sheet to determine the number of wells to be used and put any remaining wells and the desiccant back into the pouch and seal the ziploc, store unused wells at 4°C.
3. Add 100μl of standard and sample per well. Cover with the adhesive strip provided. Incubate for 2 hours at 37°C. A plate layout is provided to record standards and samples assayed.
4. Remove the liquid of each well, don't wash.
5. Add 100μl of Biotin-antibody (1x) to each well. Cover with a new adhesive strip. Incubate for 1 hour at 37°C. (Biotin-antibody (1x) may appear cloudy. Warm up to room temperature and mix gently until solution appears uniform.)
6. Aspirate each well and wash, repeating the process two times for a total of three washes. Wash by filling each well with Wash Buffer (200μl) using a squirt bottle, multi-channel pipette, manifold dispenser, or autowasher, and let it stand for 2 minutes, complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining wash Buffer by aspirating ordecanting. Invert the plate and blot it against clean paper towels.
7. Add 100μl of HRP-avidin (1x) to each well. Cover the microtiter plate with a new adhesive strip. Incubate for 1 hour at 37°C.
8. Repeat the aspiration/wash process for five times as in step 6.
9. Add 90μl of TMB Substrate to each well. Incubate for 15-30 minutes at 37°C. Protect from light.
10. Add 50μl of Stop Solution to each well, gently tap the plate to ensure thorough mixing.
11. Determine the optical density of each well within 5 minutes, using a microplate reader set to 450 nm. If wavelength correction is available, set to 540 nm or 570 nm. Subtract readings at 540 nm or 570 nm from the readings at 450 nm. This subtraction will correct for optical imperfections in the plate. Readings made directly at 450 nm without correction may be higher and less accurate.
|Calculation of results:||Using the professional soft "Curve Expert 1.3" to make a standard curve is recommended, which can be downloaded from our web.
Average the duplicate readings for each standard and sample and subtract the average zero standard optical density.
Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the CASP3 concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. This procedure will produce an adequate but less precise fit of the data.
If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
|Title||Etiology of autistic features: the persisting neurotoxic effects of propionic acid|
|Type of publication||Product-specific|
|Journal||Journal of Neuroinflammation|