Purity
>96% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The biological activity determined by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide is in a concentration range of 0.1-5.0 μg/ml, after pretreating with rHuTIGAR-TAT for 4 hours.
Research Area
Cell Biology
Alternative Names
6-bisphosphatase TIGAR; C12ORF5; chromosome 12 open reading frame 5; FR2BP; Fructose-2,6-bisphosphatase TIGAR; Fructose-2,6-bisphosphate 2-phosphatase; Probable fructose 2,6 bisphosphatase TIGAR; Probable fructose-2; tigar; TIGAR_HUMAN; TP53 induced glycolysis and apoptosis regulator; TP53 induced glycolysis regulatory phosphatase; TP53-induced glycolysis and apoptosis regulator; Transactivated by NS3TP2 protein
Species
Homo sapiens (Human)
Expression Region
1-270aa
Complete Sequence
MARFALTVVRHGETRFNKEKIIQGQGVDEPLSETGFKQAAAAGIFLNNVKFTHAFSSDLMRTKQTMHGILERSKFCKDMTVKYDSRLRERKYGVVEGKALSELRAMAKAAREECPVFTPPGGETLDQVKMRGIDFFEFLCQLILKEADQKEQFSQGSPSNCLETSLAEIFPLGKNHSSKVNSDSGIPGLAASVLVVSHGAYMRSLFDYFLTDLKCSLPATLSRSELMSVTPNTGMSLFIINFEEGREVKPTVQCICMNLQDHLNGLTETR+GGYGRKKRRQ
Protein Length
Full Length
Tag Info
C-terminal TAT-tagged
Buffer
Lyophilized from a 0.2 m filtered 30 % Acetonitrile, 0.1% TFA
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
To generate a recombinant human fructose-2,6-bisphosphatase TIGAR protein in E. coli, the full-length human TIGAR gene is inserted into an expression vector containing a C-terminal TAT-tag. The recombinant plasmid is then transformed into E. coli cells for protein production. Following expression, the cells are harvested, and the recombinant TIGAR protein is purified the cell lysate. The purity of the recombinant TIGAR protein exceeds 96% as determined by both SDS-PAGE and HPLC analysis. The activity of the recombinant TIGAR protein is assessed by its ability to protect U2OS cells from apoptosis induced by hydrogen peroxide. After pretreatment with the purified rHuTIGAR-TAT protein for 4 hours, the concentration range of 0.1-5.0 μg/ml demonstrates this recombinant TIGAR protein's efficacy in preserving cell viability. Its endotoxin is less than 1.0 EU/μg as determined by the LAL method.