KRT19 Monoclonal Antibody

Code CSB-MA0125342A0m
Size US$210
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  • All lanes:Mouse anti-Human Cyfra21-1(KS19.1) monoclonal antibody at 4ug/ml
    Lane 1: MCF-7 cell lysate
    Lane2: MDA-MB-231 cell lysate
    Lane3: HepG2 cell lysate
    Secondary HRP labeled Goat polyclonal to Mouse IgG at 1/3000 dilution
    Predicted band size : 30 kDa
    Observed band size : 32 kDa
    Additional bands at: 50 kDa

  • Immunohistochemical of paraffin-embedded human prostate tissue using CSB-MA0125342A0m at dilution of 1:200

  • Immunohistochemical of paraffin-embedded human esophageal tissue using CSB-MA0125342A0m at dilution of 1:200

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Product Details

Alternative Names
KRT19,CK19
Raised in
mouse
Species Reactivity
Human
Immunogen
Cyfra21-1 (KS19.1)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
monoclonal
Isotype
IgG2a
Clone No.
2B1D6
Purification Method
>95%,Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 15mmol/L NaN3Constituents: 0.1mol/L Nacl
Form
liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:50-1:500
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The KRT19 monoclonal antibody is produced by immunizing mice with human Cyfra21-1 (KS19.1) protein, followed by the isolation of B cells from the immunized mice spleens and the fusion of these B cells with myeloma cells to generate hybridomas. The hybridomas are then screened to select those that produce the KRT19 monoclonal antibody. The selected hybridomas are cultured in a mouse abdominal cavity to generate a large quantity of the KRT19 monoclonal antibody. The specificity of the KRT19 monoclonal antibody is confirmed by its ability to react with human KRT19 protein in three applications ELISA, WB, and IHC. It is purified using protein G affinity chromatography, resulting in a purity of over 95%.

KRT19 mainly functions to provide structural support to epithelial cells and tissues, contributing to their integrity and resilience. KRT19 plays a role in cell signaling and is involved in regulating cell proliferation and differentiation, as well as protecting cells from oxidative stress.

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