Recombinant Human herpesvirus 8 type P protein (VMI2), partial (Active)

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Code CSB-AP001611HKE
MSDS
Size $142
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Product Details

Purity
>97% as determined by SDS-PAGE.
Endotoxin
Less than 1.0 EU/μg as determined by LAL method.
Activity
Fully biologically active when compared to standard. The specific activity is determined by the inhibitory effect on monocyte migration response to human MIP-1 alpha using a concentration range of 1.0ug-10.0ug/ml of viral MIP-2 will inhibit 25ng/ml of human MIP-1 alpha.
Target Names
ORF
Uniprot No.
Research Area
Immunology
Alternative Names
ORF; K4; Viral macrophage inflammatory protein 2; Viral macrophage inflammatory protein II; vMIP-II; vMIP-1B
Species
Human herpesvirus 8 type P (isolate GK18) (HHV-8) (Kaposi\'s sarcoma-associated herpesvirus)
Source
E.coli
Expression Region
24-93aa
Complete Sequence
LGASWHRPDK CCLGYQKRPL PQVLLSSWYP TSQLCSKPGV IFLTKRGRQV CADKSKDWVK KLMQQLPVTA
Mol. Weight
8.0 kDa
Protein Length
Partial
Tag Info
Tag-Free
Form
Lyophilized powder
Buffer
Lyophilized from a 0.2 µm filtered PBS, pH 7.4
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
5-10 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Blocks infection by several different human immunodeficiency virus type 1 (HIV-1) strains. This occurs because vMIP-II binds to a wide range of chemokine receptors. May form part of the response to host defenses contributing to virus-induced neoplasia and may have relevance to KSHV and HIV-I interactions.
Gene References into Functions
  1. this study reports the crystal structure of the chemokine receptor CXCR4 in complex with the viral chemokine antagonist vMIP-II at 3.1 angstrom resolution. PMID: 25612609
  2. A dimeric vMIP-II mutant binds to heparin much more tightly than wild-type vMIP-II. PMID: 20712376
  3. vMIP-II forms crystallographic dimers in our tetragonal crystal through interactions between N-termini, which is the interaction pattern also observed in the asymmetric unit of previously reported crystal form. PMID: 17243149
  4. vCCL2 was found to act as a broad spectrum chemokine antagonist of human chemokine receptors, including the lymphotactin receptor PMID: 17403668
Subcellular Location
Secreted.
Protein Families
Intercrine beta (chemokine CC) family
Database Links

KEGG: vg:4961514

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