FARP1 Antibody

Datasheet

Code	CSB-PA897492LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: A375 whole cell lysate, HEK293 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: FARP1 antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 119, 123, 15 kDa Observed band size: 119 kDa Immunohistochemistry of paraffin-embedded human liver tissue using CSB-PA897492LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human liver cancer using CSB-PA897492LA01HU at dilution of 1:100 Immunofluorescent analysis of HepG2 cells using CSB-PA897492LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) Immunofluorescent analysis of A549 cells using CSB-PA897492LA01HU at dilution of 1:100 and Cy3-congugated Goat Anti-Rabbit IgG
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) FARP1 Polyclonal antibody

Uniprot No.

Q9Y4F1

Target Names

FARP1

Alternative Names

CDEP antibody; Chondrocyte derived ezrin like protein antibody; Chondrocyte-derived ezrin-like protein antibody; FARP1 antibody; FARP1_HUMAN antibody; FERM RhoGEF and pleckstrin domain containing protein 1 antibody; FERM antibody; FERM RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) antibody; FERM; ARHGEF; and pleckstrin domain protein 1 antibody; PH domain-containing family C member 2 antibody; Pleckstrin homology domain-containing family C member 2 antibody; PLEKHC2 antibody; PPP1R75 antibody; protein phosphatase 1; regulatory subunit 75 antibody; RhoGEF and pleckstrin domain-containing protein 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human FERM, ARHGEF and pleckstrin domain-containing protein 1 protein (1-300AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The FARP1 Antibody (Product code: CSB-PA897492LA01HU) is Non-conjugated. For FARP1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA897492LB01HU	FARP1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA897492LC01HU	FARP1 Antibody, FITC conjugated
Biotin	CSB-PA897492LD01HU	FARP1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The generation of the product CSB-PA897492LA01HU is through immunizing rabbits with the peptide corresponding to amino acids 1-300 of the recombinant human FARP1 protein. The production is a polyclonal antibody and occurs as an unconjugated IgG. It is reactive with human FARP1 protein, which participates in regulating neuronal development through interacting with cell-surface proteins such as class A Plexins and SynCAM 1. Its purity is greater than 95% using protein G purified. And this FARP1 antibody has been validated for use in ELISA, WB, IHC, and IF applications.

Customer Reviews and Q&A

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Target Background

Function

Functions as guanine nucleotide exchange factor for RAC1. May play a role in semaphorin signaling. Plays a role in the assembly and disassembly of dendritic filopodia, the formation of dendritic spines, regulation of dendrite length and ultimately the formation of synapses.

Gene References into Functions

We have reported here for the first time a reduced activity of both Rac1 and Cdc42 in human pheochromocytoma resection as well as tumor-associated expression changes of FARP1, ARHGEF1, and ARHGAP36 PMID: 26911374

Subcellular Location

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell junction, synapse, synaptosome. Cytoplasm, cytosol. Cell projection, filopodium. Cell projection, dendrite. Cell projection, dendritic spine.

Tissue Specificity

Detected in cAMP-treated chondrocytes, but not in untreated chondrocytes. Detected in fetal brain, heart and spleen, and in adult testis, kidney and lung.

Database Links

HGNC: 3591

OMIM: 602654

KEGG: hsa:10160

STRING: 9606.ENSP00000322926

UniGene: Hs.403917

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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