Code | CSB-PA003500 |
Size | US$100 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:2000 |
IHC | 1:100-1:300 |
ELISA | 1:20000 |
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Could you please provide more info on the recommended dilution solvent and how to be reconstituted?
We recommend 1*PBS as dilution solvent, the formula of PBS :
0.01M PBS: PBS buffer (pH 7.2~7.4): NaCl 137mmol/L, KCL 2.7mmol/L, Na2HPO4 4.3mmol/L, KH2PO4 1.4mmol/L
Weight 7.9 g of NaCl, 0.2g of KH2PO4 ( or 1.44g Na2HPO4) and 1.8g K2HPO4, dissolve in 800ml of sterilized water.
Use HCl to adjust the solution pH value to 7.4, and then add sterilized water to 1L, store at 4℃.