PDK2 Antibody

Code CSB-PA618976ESR2HU
Size US$166
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  • Western blot
    All lanes: PDK2 antibody at 6μg/ml
    Lane 1: Mouse heart tissue
    Lane 2: Hela whole cell lysate
    Lane 2: Mouse skeletal muscle tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 47, 39 kDa
    Observed band size: 47 kDa

  • Immunohistochemistry of paraffin-embedded human berast cancer using CSB-PA618976ESR2HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human skeletal muscle tissue using CSB-PA618976ESR2HU at dilution of 1:100

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) PDK2 Polyclonal antibody
Uniprot No.
Target Names
PDK2
Alternative Names
[Pyruvate dehydrogenase [lipoamide]] kinase isozyme 2 antibody; mitochondrial antibody; PDHK2 antibody; PDK2 antibody; PDK2_HUMAN antibody; Pyruvate dehydrogenase kinase isoform 2 antibody; Pyruvate dehydrogenase kinase, isozyme 2 antibody; Pyruvate dehydrogenase lipoamide kinase isozyme 2, mitochondrial antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 2, mitochondrial protein (128-407AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Form
Liquid
Tested Applications
ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Kinase that plays a key role in the regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Inhibition of pyruvate dehydrogenase decreases glucose utilization and increases fat metabolism. Mediates cellular responses to insulin. Plays an important role in maintaining normal blood glucose levels and in metabolic adaptation to nutrient availability. Via its regulation of pyruvate dehydrogenase activity, plays an important role in maintaining normal blood pH and in preventing the accumulation of ketone bodies under starvation. Plays a role in the regulation of cell proliferation and in resistance to apoptosis under oxidative stress. Plays a role in p53/TP53-mediated apoptosis.
Gene References into Functions
  1. PDK2/PARL senses defects in mitochondrial bioenergetics. PMID: 28178523
  2. The data demonstrate potential roles of PDK2 and ABCG2 polymorphisms in the metabolic phenotypes of Tibetan gout patients. PMID: 26909964
  3. High glycolysis and PDK2 overexpression are closely linked to cisplatin resistance in head and neck cancer cells, which can be reversed by PDK2 nhibition. PMID: 26607904
  4. The findings of the present study reveal a novel survival pathway that functionally couples the unique glycolytic phenotype in cancer cells to hypoxia resistance via a PDK2-dependent mechanism that switches Bnip3 from cell death to survival. PMID: 26416963
  5. The final compound of this series, 2-[(2,4-dihydroxyphenyl)sulfonyl]isoindoline-4,6-diol, designated PS10, inhibits all four PDK isoforms with IC50 = 0.8 muM for PDK2. PMID: 24356970
  6. Germline mutations in PKD2 gene is associated with autosomal-dominant polycystic kidney disease. PMID: 23300259
  7. we for the first time demonstrated that a low-nutrient condition drives cancer cells to utilize glycolysis to produce ATP, and this increases the Warburg effect through a novel mechanism involving ROS/AMPK-dependent activation of PDK. PMID: 23376776
  8. Mitochondrial activation by inhibition of PDKII suppresses HIF1a signaling and angiogenesis in cancer. PMID: 22614004
  9. Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species. PMID: 22910903
  10. Results established that wild-type p53 prevents manifestation of the Warburg effect by controlling Pdk2. These findings elucidate a new mechanism by which p53 suppresses tumorigenesis acting at the level of cancer cell metabolism. PMID: 22123926
  11. PKD2 mutations are associated with autosomal dominant polycystic kidney disease. PMID: 21115670
  12. study of facilitated interaction between the pyruvate dehydrogenase kinase isoform 2 and the dihydrolipoyl acetyltransferase PMID: 12816949
  13. The increased PDK activity was independent of changes in intra-mitochondrial effectors, and PDK-2 and PDK-4 protein content, suggesting that it was caused by a change in the specific activity of the existing kinases. PMID: 15169745
  14. A mechanism for pyruvate dehydrogenase kinase isoform 2 regulation is supported in which kinase activity is limited by ADP dissociation and pyruvate binding. PMID: 15491150
  15. Reductive acetylation stimulates activity of pyruvate dehydrogenase kinase isoform 2 by speeding up ADP dissociation. PMID: 15491151
  16. in human muscle, hormonal and nutritional conditions may control PDK2 and PDK4 mRNA expression via a common signalling mechanism. PMID: 15955060
  17. Crystallographic studies reveal several PDHK2 structures with C-terminal cross arms that span a large trough region between the N-terminal regulatory (R) domains of the PDHK2 dimers. Three novel ligand binding sites are located in the R domain of PDHK2. PMID: 16401071
  18. analysis of ligand induced effects on pyruvate dehydrogenase kinase isoform 2 PMID: 16517984
  19. These studies identify ORP9 as a PDK-2 substrate and negative regulator of Akt phosphorylation at the PDK-2 site. PMID: 16962287
  20. PDK2, PDK3 and PDK4 are primary PPARbeta/delta target genes in humans underlining the importance of the receptor in the control of metabolism PMID: 17669420
  21. Critical role of specific ions for ligand-induced changes regulating pyruvate dehydrogenase kinase isoform 2. PMID: 18220414
  22. Pi is suggested to facilitate transmission within PDHK2 of the stimulatory signal of acetylation from the distal lipoyl-group binding site to the active site. PMID: 18220415
  23. mitochondrial ND2 mutation contributes to HIF1alpha accumulation via increased ROS production, up-regulation of PDK2, attenuating PDH activity, thereby increasing pyruvate, resulting in HIF1alpha stabilization PMID: 19147752
  24. PKD1 children have more and larger renal cysts, larger kidneys and higher ambulatory BP than do PKD2 children. PMID: 19194729
  25. the DW-motif has a pivotal role in mediating communications between the DCA-, the nucleotide-, and the lipoyl domain-binding sites of PDK2 PMID: 19833728

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Subcellular Location
Mitochondrion matrix.
Protein Families
PDK/BCKDK protein kinase family
Tissue Specificity
Expressed in many tissues, with the highest level in heart and skeletal muscle, intermediate levels in brain, kidney, pancreas and liver, and low levels in placenta and lung.
Database Links

HGNC: 8810

OMIM: 602525

KEGG: hsa:5164

STRING: 9606.ENSP00000420927

UniGene: Hs.256667

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