Cusabio
Product Name:

Human HSD11B1 Primer Pair 2

Product Type:
RT-PCR Primers
Code:
CSB-PP147321h
Size:
25T
Description:
Human HSD11B1 Primer Pair 2 for RT-PCR (reverse transcription followed by polymerase chain reaction) analysis of mRNA expression.
Length:
804bp
Accession NO.:
NM_005525
Application ares:
RT-PCR
Storage:
The Primer Pair is stable for up to one year at -20° C in a non-frost free freezer. Avoid repeated freeze-thaw cycles.
Quality Control:
Primer Pairs for RT-PCR provide convenient primers for analyzing the expression of specific mRNAs or got specific PCR fragment by RT-PCR. Each Primer Pair includes a 5´ and 3´ oligonucleotide primer provided in individual vials at 50μM ready for addition to the amplification reaction. The Primer Pairs have been confirmed in two-step conventional RT-PCR using human hepatic L02 cells or human hepatocellular carcinoma HepG2 cells total RNA. PCR products from the use of the Primer Pairs have been sequence-confirmed for appropriate.
Technical Hints:
• Thaw all reagents completely on ice before use. • Use either random primers or oligo (dT) for reverse transcription. • All PCR reactions should be assembled on ice. • The recommended annealing
Protocol:

Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.