Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
CCapsid protein; Core antigen; Core protein; HBcAg; p21.5
Species
Hepatitis B virus genotype C subtype ayw (isolate China/Tibet127/2002) (HBV-C)
Expression Region
1-183aa
Target Protein Sequence
MDIDPYKEFGASVEVLSFLPSDFFPSNRDLLDTASALDREALESPEHCSPHHTALRQAILCWGELMNLATWVGSNLEDPASRELVVSYVNVNMGLKIRQLLWFHISCLTFGRETVLEYLVSFGVWIRTPPAYRPPNAPILSTLPETTVVRRRGRSPRRRTPSPRRRRSQSPRRRRSQSRESQC
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
CUSABIO transfected the expression vector which inserted the recombinant DNA into the yeast, cultured the cells, and then induced the transcription and translation of the cloned vector. The N-terminal 6xHis tag sequence was appended to the gene coding for the yeast of the HBV-C Capsid protein (C) protein to form the recombinant DNA. The recombinant HBV-C Capsid protein (C) was expressed as N-terminal 6xHis-tagged fusion. The purity of the protein is greater than 90% assayed by SDS-PAGE. It has an apparent molecular weight of approximately 22 kDa.
Hepatitis B virus is an important human pathogen. HBV encodes a 21 kDa core protein, which is required for reverse transcription. This core protein can assemble into stable icosahedral particles, when the electrostatic interactions between positively and negatively charged macromolecules at the inner surface of the capsid shell are adequately balanced. It remains unclear what determines the subcellular localization of hepatitis B virus (HBV) core protein (HBc) and particles. Chronic infection with hepatitis B virus HBV could lead to cirrhosis and highly malignant liver cancer. At present, treatment of hepatitis B is not very effective, due to notorious side effects and drug resistance. The virus can synthesize a core protein for its own replication. Clinically, this core protein tends to be more localized to the cytoplasm when patients' disease is severe, and more localized to the nucleus when the disease is mild.
