Recombinant Human Clathrin interactor 1 (CLINT1)

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Code CSB-EP005552HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
CLINT1
Uniprot No.
Research Area
Signal Transduction
Alternative Names
Clathrin interacting protein; Clathrin interactor 1; Clathrin-interacting protein localized in the trans-Golgi region; CLINT 1; Clint; CLINT1; ENTH; Enthoprotin; EPN4; EPN4_HUMAN; Epsin 4; Epsin related protein; Epsin-4; Epsin-related protein; EpsinR
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-625aa
Target Protein Sequence
MLNMWKVRELVDKATNVVMNYSEIESKVREATNDDPWGPSGQLMGEIAKATFMYEQFPELMNMLWSRMLKDNKKNWRRVYKSLLLLAYLIRNGSERVVTSAREHIYDLRSLENYHFVDEHGKDQGINIRQKVKELVEFAQDDDRLREERKKAKKNKDKYVGVSSDSVGGFRYSERYDPEPKSKWDEEWDKNKSAFPFSDKLGELSDKIGSTIDDTISKFRRKDREDSPERCSDSDEEKKARRGRSPKGEFKDEEETVTTKHIHITQATETTTTRHKRTANPSKTIDLGAAAHYTGDKASPDQNASTHTPQSSVKTSVPSSKSSGDLVDLFDGTSQSTGGSADLFGGFADFGSAAASGSFPSQVTATSGNGDFGDWSAFNQAPSGPVASSGEFFGSASQPAVELVSGSQSALGPPPAASNSSDLFDLMGSSQATMTSSQSMNFSMMSTNTVGLGLPMSRSQNTDMVQKSVSKTLPSTWSDPSVNISLDNLLPGMQPSKPQQPSLNTMIQQQNMQQPMNVMTQSFGAVNLSSPSNMLPVRPQTNALIGGPMPMSMPNVMTGTMGMAPLGNTPMMNQSMMGMNMNIGMSAAGMGLTGTMGMGMPNIAMTSGTVQPKQDAFANFANFSK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
72.3kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

I am looking at CSB-EP005552HU Recombinant Human Clathrin interactor 1(CLINT1) for a recombinant protein produced from E. coli.
I am wondering what's the phosphorylation state of your recombinant proteins? I want to use it as substrate in kinase assay so it's crucial to have these proteins in non-phosphorylated state.
Could you tell me if you had phosphatase in the bacteria when you purified the protein?

A:
Thanks for your inquiry,CSB-EP005552HU This protein is sourced from E. coli recombinant expression system, which belongs to Prokaryotic expression and lack of post-translation modification. So this protein should be in non-phosphorylation state in theory.

Target Background

Function
Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May have a role in transport via clathrin-coated vesicles from the trans-Golgi network to endosomes. Stimulates clathrin assembly.
Gene References into Functions
  1. Although epsinR has been shown to be a cargo adaptor, the present study indicates that it is an order of magnitude more abundant in CCVs than its known cargo protein, vti1b, suggesting that it may have other functions as well. PMID: 26179914
  2. These results do not support a significant role of GRIA1 or CLINT1 in the development of schizophrenia in the German population. PMID: 21116212
  3. a novel clathrin-associated protein identified through subcellular proteomics; data suggest a role in clathrin-mediated budding on internal membranes PMID: 12213833
  4. might participate in the formation of clathrin-coated vesicles at the level of the trans-Golgi network and remains associated with the vesicles longer than clathrin and adaptors PMID: 12429846
  5. EpsinR is an ENTH domain-containing protein that interacts with AP-1 PMID: 12589059
  6. Our work reveals the existence of clathrin-independent and -dependent transport steps in the retrograde route, and establishes a function for clathrin and epsinR at the endosome-TGN interface. PMID: 15068792
  7. epsinR is an adaptor for vesicle-associated soluble NSF attachment protein receptor PMID: 15371541
  8. Four adjacent markers at the 5' end of the Epsin 4 gene showed significant evidence of linkage disequilibrium with schizophrenia. PMID: 15793701
  9. Results indicate the presence of a locus near the 5' end of Epsin 4 conferring susceptibility to the disease and provide further support for Epsin 4 as an important potential contributor to genetic risk in schizophrenia. PMID: 16402136
  10. characterization of the molecular details governing the sorting of a SNARE into clathrin-coated vesicles, namely the direct recognition of the three-helical bundle H(abc) domain of the mouse SNARE Vti1b by the human clathrin adaptor epsinR PMID: 18033301
  11. Our results suggest that the examined region of Epsin 4 does not have a major influence on susceptibility to schizophrenia in Japanese. PMID: 18696005
  12. Variation in the Epsin 4 gene is significantly associated with psychotic disorder in this Latino population PMID: 18929466

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Subcellular Location
Cytoplasm. Cytoplasm, perinuclear region. Membrane; Peripheral membrane protein. Cytoplasmic vesicle, clathrin-coated vesicle. Note=Found throughout the cell, with the exception of the cell surface. Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the trans-Golgi network.
Protein Families
Epsin family
Tissue Specificity
Ubiquitously expressed at low to intermediate levels.
Database Links

HGNC: 23186

OMIM: 607265

KEGG: hsa:9685

STRING: 9606.ENSP00000429824

UniGene: Hs.644000

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