Recombinant Mouse Glycosyltransferase 54 domain-containing protein

Code CSB-YP861542MO
MSDS
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Source Yeast
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Code CSB-EP861542MO
MSDS
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Source E.coli
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Code CSB-EP861542MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861542MO
MSDS
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Source Baculovirus
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Code CSB-MP861542MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mgat4d
Uniprot No.
Alternative Names
Mgat4dAlpha-1,3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase-like protein MGAT4D; N-acetylglucosaminyltransferase MGAT1 inhibitory protein; GlcNAcT-I inhibitory protein; GnT1IP
Species
Mus musculus (Mouse)
Expression Region
27-373
Target Protein Sequence
RMNQ TNNQLINCRN HVLEFKEIML RLKNKSENHH QDLMQVLYQM KRKAAHTTRS SGNFLEKKGS ILSQHETLPN QFEVLKYFLP HLRTAGKLYP AIATSKGRAG VSFALGISTI NRGNHTYLKQ TLTSVLSRMT PEEEEDSVVI VSVADTDESY LKSVVRMVKT KFRKQVQSGV LEVISIPTLF YPQTLLDKKT KTDSESWQIK QVLDFCILML YAQPKATYYL QLEDDIVAKK MYFTKMKDFV NSLTSKNWFF IEFSVLGFIG KLFRSKDLTD FVHFFLMFYE TKPIDILLDD IFLIRVCISG EPVRSCLQRK KGFRIQYRPS LFQHVGTQSS FPGREQHLKD NYY
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May play a role in male spermatogenesis. In vitro acts as inhibitor of MGAT1 activity causing cell surface proteins to carry mainly high mannose N-glycans. The function is mediated by its lumenal domain and occurs specifically in the Golgi. A catalytic glucosyltransferase activity is not detected. May be involved in regulation of Sertoli-germ cell interactions during specific stages of spermatogenesis.
Subcellular Location
[Isoform 2]: Endoplasmic reticulum membrane; Single-pass type II membrane protein. Endoplasmic reticulum-Golgi intermediate compartment membrane; Single-pass type II membrane protein. Golgi apparatus membrane; Single-pass type II membrane protein.; [Isoform 1]: Golgi apparatus membrane; Single-pass type II membrane protein.
Protein Families
Glycosyltransferase 54 family
Tissue Specificity
Isoform 1 and isoform 2 are specifically expressed in testis. Isoform 2 is expressed in spermatocytes but not in spermatids. Isoform 1 is expressed in spermatids.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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