Name: Recombinant Human Pro-neuregulin-3, membrane-bound isoform (NRG3), partial
Brand: CUSABIO
SKU: CSB-EP016079HU

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

NRG3

Uniprot No.

P56975

Research Area

Cancer

Alternative Names

HRG3; Neuregulin 3; Neuregulin 3 like polypeptide; Neuregulin-3; Neuregulin3; NRG-3; Nrg3; NRG3_HUMAN; Pro neuregulin 3; pro NRG3; Pro-NRG3

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-360aa

Target Protein Sequence

MSEGAAAASPPGAASAAAASAEEGTAAAAAAAAAGGGPDGGGEGAAEPPRELRCSDCIVWNRQQTWLCVVPLFIGFIGLGLSLMLLKWIVVGSVKEYVPTDLVDSKGMGQDPFFLSKPSSFPKAMETTTTTTSTTSPATPSAGGAASSRTPNRISTRLTTITRAPTRFPGHRVPIRASPRSTTARNTAAPATVPSTTAPFFSSSTLGSRPPVPGTPSTQAMPSWPTAAYATSSYLHDSTPSWTLSPFQDAASSSSSSSSSATTTTPETSTSPKFHTTTYSTERSEHFKPCRDKDLAYCLNDGECFVIETLTGSHKHCRCKEGYQGVRCDQFLPKTDSILSDPTDHLGIEFMESEEVYQRQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

53.8kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human NRG3 was expressed with the amino acid range of 1-360. The expected molecular weight for the NRG3 protein is calculated to be 53.8 kDa. Expression of this NRG3 protein is conducted in e.coli. The NRG3 coding gene included the N-terminal 6xHis-SUMO tag, which simplifies the detection and purification processes of the recombinant NRG3 protein in following stages of expression and purification.

The human pro-neuregulin-3 is a membrane-bound isoform of the neuregulin-3 (NRG3) protein. Neuregulins are signaling proteins involved in neural development and function. NRG3 plays a crucial role in neuronal and glial differentiation, as well as in the development of the nervous system. As a membrane-bound isoform, pro-neuregulin-3 likely functions in cell-cell communication by interacting with its receptors on neighboring cells. Research areas related to pro-neuregulin-3 include neurobiology, neurodevelopmental disorders, and potential implications in conditions affecting the nervous system. Understanding its role may provide insights into the molecular mechanisms underlying neural development and contribute to therapeutic strategies for neurological disorders.

Customer Reviews and Q&A

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■ Q&A

May I ask you for support in the following inquiry please?
It concerns the human recombinant Neuregulin proteins NRG2 and NRG3 (Cat# CSB-EP016079HU).
I would like to stimulate human cells in culture with these ligands, and check downstream signaling by immunoprecipitation and western blotting of target proteins. At the moment the source organism is probably irrelevant as long as the ligands are active when used for stimulation of cells in culture.
Have you done any such tests with these growth factors?

Very nice to receive your inquiry. Code: CSB-EP016079HU
Name: Recombinant Human Pro-neuregulin-3, membrane-bound isoform(NRG3),partial
Expression Region: 1-360aa, extracellular domain
Tag Info: N-terminal 6xHis-SUMO-tag
Target Protein Sequence:

We haven't tested the activity of this protein when it is used as ligand, and haven't found relevant literature reporting the activity of exogenous expression protein.
Here we provide the literature regarding this isolated protein's structure and function:
http://www.uniprot.org/uniprot/P56975/publication

Target Background

Function

Direct ligand for the ERBB4 tyrosine kinase receptor. Binding results in ligand-stimulated tyrosine phosphorylation and activation of the receptor. Does not bind to the EGF receptor, ERBB2 or ERBB3 receptors. May be a survival factor for oligodendrocytes.

Gene References into Functions

No genetic correlation was found between Hirschsprung disease and the three SNPs at NRG3 (rs10748842, rs10883866 and rs6584400) PMID: 28256518
Data show that Williams-Beuren syndrome transcription factor (WSTF) release was mediated by neuregulin-3 (NRG3) following KRASG12V expression in intestinal epithelial cells. PMID: 27449290
These observations encourage investigation of the neurobiology of NRG3 isoforms and highlight inhibition of NRG3 signaling as a potential target for psychiatric treatment development. PMID: 27771971
common variants of GABRG2, RELN and NRG3 and the GABRG2-RELN-PTCH1 interaction networks might confer altered susceptibility to Hirschsprung disease. PMID: 27889765
these data suggest that Nrg3/ErbB4 signaling may be an important factor in nicotine dependence. PMID: 23999525
This study demonstrated that genetic variants in the NRG3 gene play a role in alzheimer disease and revealed that SNPs in the NRG3 genes were more strongly associated with onset age of Alzherimer disease. PMID: 24061483
An association between rs10748842 genotype in neuregulin 3 does not fit a simple inefficiency model of risk association in dorsolateral prefrontal cortex physiology. PMID: 24431462
NRG3 may be a susceptibility gene for Hirschsprung's disease in a Chinese population. PMID: 23315268
Neuregulin 3 rs6584400 genetic carriers are associated with psychotic symptoms and attention performance in schizophrenia. PMID: 22831755
no association of SNPs or haplotypes with risk of schizophrenia in Han Chinese population PMID: 22981155
Our results suggest a role for NRG3 in HSCR etiology and provide insights into the relative contribution of structural variants in both syndromic and non-syndromic HSCR. PMID: 22589734
NRG3 polymorphisms and haplotypes were similar between schizophrenia patients and healthy controls of Korean ethnicity PMID: 21762460
Data show that rs1649942, which is located in the intron region of NM_001165973.1. (NRG3 neuregulin 3), was associated with poorer overall survival in women with optimally debulked tumors. PMID: 21705454
Results suggest that NRG3 may be modulating early attentional processes for perceptual sensitivity and vigilance, with opposite effects in affected individuals and healthy controls. PMID: 20548296
Data show that NRG3 is a schizophrenia susceptibility gene, provide quantitative insight into NRG3 transcription traits in the brain, and reveal a probable mechanistic basis for disease association. PMID: 20713722
The NRG3 protein is a new post-translationally regulated isoform of neuregulin 3 expressed in the developing human central nervous system with a role in oligodendrocyte survival. PMID: 16478787
In transgenic female mice, Nrg3 has a role in the initiation of mammary placodes along the body axis; alterations in morphogenesis and differentiation of other epidermal appendages are also observed, including the hair follicles. PMID: 17880691
NRG3 was expressed in 42% of breast cancers studied. It was preferentially expressed in breast tumors co-expressing EGFR/HER4. PMID: 17962208
The result of tis study supports that the NRG3 gene is a susceptibility gene for schizophrenia. PMID: 18708184
NRG3 is primarily expressed in the central nervous system and is one of three paralogs of NRG1, a gene strongly implicated in SZ. PMID: 19118813
Observational study and genome-wide association study of gene-disease association and gene-environment interaction. (HuGE Navigator) PMID: 18846501

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Subcellular Location

[Pro-neuregulin-3, membrane-bound isoform]: Cell membrane; Single-pass type I membrane protein.; [Neuregulin-3]: Secreted.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein. Note=Isoform 3 is also proteolytically released as a soluble form.

Protein Families

Neuregulin family

Tissue Specificity

Highly expressed in most regions of the brain with the exception of corpus callosum. Expressed at lower level in testis. Not detected in heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, ovary, small intestine, col

Database Links

HGNC: 7999

OMIM: 605533

KEGG: hsa:10718

STRING: 9606.ENSP00000361214

UniGene: Hs.125119

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