Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
ApoeApolipoprotein E; Apo-E
Species
Mus musculus (Mouse)
Expression Region
19-311aa
Target Protein Sequence
EGEPEVTDQLEWQSNQPWEQALNRFWDYLRWVQTLSDQVQEELQSSQVTQELTALMEDTMTEVKAYKKELEEQLGPVAEETRARLGKEVQAAQARLGADMEDLRNRLGQYRNEVHTMLGQSTEEIRARLSTHLRKMRKRLMRDAEDLQKRLAVYKAGAREGAERGVSAIRERLGPLVEQGRQRTANLGAGAAQPLRDRAQAFGDRIRGRLEEVGNQARDRLEEVREHMEEVRSKMEEQTQQIRLQAEIFQARLKGWFEPIVEDMHRQWANLMEKIQASVATNPIITPVAQENQ
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Apoe/Apolipoprotein E Protein CSB-EP001936MO is a Recombinant Mouse Apoe, which is the full-length mature protein from amino acid 19 to 311. It is produced in E.coli through Prokaryotic expression and fused with a 6xHis-tag at the N-terminus. And it has high purity of up to 90% as determined by SDS-PAGE. Its predicted molecular weight is 38.0 kDa, but the actual observed molecular mass via SDS-PAGE analysis is a little bit more than 38 kDa due to post-transcriptional modifications such as glycosylation. Large stock of this recombinant Apoe protein allows for continuous sourcing and no intermediate waiting period for protein preparation. This Apoe protein not only acts as an immunogen for antibody production but also find uses on the studies of neuronal signal transduction and neurodegenerative diseases.
Apoe is a polymorphic lipoprotein that predominantly mediates the binding of lipoproteins or lipid complexes in the plasma or interstitial fluids to specific cell-surface receptors and subsequent internalization and catabolism of the Apoe-containing lipoprotein particles. It is thus involved in the distribution/distribution of lipids among various tissues and cells of the body. It also exerts multiple other cellular functions such as glucose metabolism, neuronal signaling, neuroinflammation, and mitochondrial function. In terms of diseases, it is closely linked to the pathogenesis of Alzheimer's disease (AD).