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cusabio

Protein

Protein

CusAb provides "No risk" custom service for millions of recombinant proteins in different species.
Features:
* No risk. No protein, No charge.
* Competitive price as low as $689/mg.
* 39 kinds of tags meeting different demands.
* Secondary AKTA-SEC purification to ensure high purity.
* Good capacity and experience for large-scale expression (10mg, 50mg, 100mg, 200mg available).
* Proteins with different sizes (5ug, 10ug, 20ug, 50ug, 100ug, 500ug, 1mg, ect).
* Multiple expression systems are available including E.coli, yeast, insect cells, mammalian cells and in vitro (cell free) expression system. (Please see 'The Characteristics of Five Expression Systems' below)

Note: This no risk custom service is only suitable for proteins within 800aa.We will charge according to different steps if you need to express proteins more than 800aa.

You can choose either way to request for the quotation:

1.

You can use Protein name / Cat No / Alias / Abbreviations / Uniprot No to search your desired protein in our database, which contain millions of proteins, then you can request the quotation through the livechat or “contact us”directly.

2.

If you can’t search the protein of your interest, please complete the online “Customized Protein Request Form”, your information will be strictly protected and will not be disclosed to the third party, we will reply you by email within 24 hours.

The Characteristics of Five Expression Systems

Escherichia coli (E.coli) expression system

Advantages

  • 1.

    Clear genetic background;

  • 2.

    Low cost and simple culture conditions;

  • 3.

    Simple transformation operation;

  • 4.

    Short protein expression period;

  • 5.

    High rate of reproduction and expression level;

  • 6.

    A variety of carriers and fusion tags to choose;

  • 7.

    Many parameters can be altered to optimize expression.

  • 8.

    Tag can be available at both N-terminal and C-terminal with an enzyme cutting site that is easy to cut.

Disadvantages

  • 1.

    Inefficient disulfide bond formation and poor folding lead to inclusion body formation;

  • 2.

    Poor post-translational modifications (such as glycosylation, alkylation, phosphorylation, specificity of proteolytic processing, etc.);

  • 3.

    The success rate and recovery rate of inclusion body renaturation is not very high.

Applications

  • Purified protein (structure, enzymology, drug discovery);
  • Protein therapeutics.
Pichia pastoris (Yeast) expression system

Advantages

  • 1.

    Low cost and high expression level;

  • 2.

    Self-produced Endotoxin-free;

  • 3.

    Efficient protein folding and secretory expression;

  • 4.

    Simple culture conditions and purification operation;

  • 5.

    Extensive post-translational modifications: Glycosylation, phosphorylation, acyl lipid;

  • 6.

    Can develop high density fermentation by using simple inorganic salt, large biomass;

  • 7.

    The protein is more stable than the prokaryotic expression, and is especially suitable for the expression of eukaryotic genes and the preparation of functional expression proteins;

  • 8.

    Simple laboratory and industrial operation.

Disadvantages

  • 1.

    Glycosylation modification is not good as mammalian cells.

Applications

  • Purified protein (structure, enzymology, drug discovery).
Baculovirus-infected insect cells expression system

Advantages

  • 1.

    Good expression levels and relatively rapid growth;

  • 2.

    More advantages over the expression of viral proteins;

  • 3.

    Glycosylation modification more like mammalian cells;

  • 4.

    Relatively de-glycosylation modification (good for structure determination);

  • 5.

    Large gene volume: because the baculovirus genome is larger, so it can carry large gene fragment;

  • 6.

    High security: the baculovirus has a strict species specificity, not infected with vertebrates and plants;

  • 7.

    High expression efficiency of exogenous gene: compared with other eukaryotic expression systems, the baculovirus system can efficiently express exogenous protein from infected cells;

  • 8.

    The expression product has high activity: baculovirus will proliferation in insect host cells, thus resulting recombinant protein will be similar to mammalian cells in post-translational modification, the expression product has a strong biological activity;

  • 9.

    Easy to amplify: baculovirus can mass production of recombinant protein products with biological activity.

Disadvantages

  • 1.

    Expensive culture media cost;

  • 2.

    Inefficient processing of pro-peptides in secretory pathway;

  • 3.

    Viral infection leads to cell lysis and potential degradation of expressed proteins;

  • 4.

    Glycosylation modification is not good as mammalian cells.

Applications

  • Purified protein (structure, enzymology, drug discovery).
Mammalian cells expression system

Advantages

  • 1.

    Self-produced Endotoxin-free;

  • 2.

    Efficient protein folding and secretory expression;

  • 3.

    All post-translational modifications, provide a variety of complex N glycosylation and accurate O type glycosylation and other post-translational processing functions;

  • 4.

    The expressed products are closest to the natural biological proteins in molecular structure, physical and chemical properties and biological functions.

Disadvantages

  • 1.

    Expensive culture media cost;

  • 2.

    Complex growth requirements.

Applications

  • Purified protein (structure, enzymology, drug discovery);
  • Protein therapeutics;
  • Cell-based studies.
Cell-free expression system

Advantages

  • 1.

    High expression level: we provide the original and optimized pET carrier with a series of labels, so that the membrane protein yield can reach mg grade;

  • 2.

    Protein synthesis conditions can be manipulated;

  • 3.

    The target fusion protein with 6*His is beneficial to the purification.

Disadvantages

  • 1.

    Limited post-translational modifications;

  • 2.

    Expensive for scale-up.

Applications

  • Purified protein (structure, enzymology, drug discovery);
  • In vitro expression cloning;
  • Isotopic labelling of proteins for NMR;
  • Incorporation of non-natural amino acids.

Protein

Advanced Search

  • Product Name Code Size
    Recombinant Plasmodium Uncharacterized protein(berghei PBANKA_093100),partial CSB-CF2221PLH 100ug/50ug/10ug
    Recombinant Rice Helix-loop-helix DNA-binding domain containing protein, expressed(LOC_Os11g32100) CSB-CF2220OGF 100ug/50ug/10ug
    Recombinant Laodelphax striatella Carboxylic ester hydrolase(CarE) CSB-CF2219 100ug/50ug/10ug
    Recombinant Gossypium hirsutum MML3_A12(MML3) CSB-CF2218GHB 100ug/50ug/10ug
    Recombinant Human Pulmonary surfactant-associated protein B(SFTPB),partial CSB-CF021173HU 100ug/50ug/10ug
    Recombinant Human Protein AF-9(MLLT3) CSB-CF014635HU 100ug/50ug/10ug
    Recombinant Human Sodium/glucose cotransporter 2(SLC5A2),partial CSB-CF021679HU 100ug/50ug/10ug
    Recombinant Arachis hypogaea Allergen Ara h 1, clone P41B CSB-CF337356ANE 100ug/50ug/10ug
    Recombinant Pig Transcobalamin-1(TCN1) CSB-CF023317PI 100ug/50ug/10ug
    Recombinant Human Progesterone-induced-blocking factor 1(PIBF1) CSB-CF845171HU 100ug/50ug/10ug
    Recombinant Human Tripartite motif-containing protein 5(TRIM5) CSB-CF874861HU 100ug/50ug/10ug
    Recombinant Human T-cell surface glycoprotein CD4(CD4),partial CSB-CF004935HU1 100ug/50ug/10ug
    Recombinant Anaplasma phagocytophilum 50S ribosomal protein L22(rplV) CSB-CF641281AAAQ 100ug/50ug/10ug
    Recombinant Human TIR domain-containing adapter molecule 1(TICAM1) CSB-CF811598HU 100ug/50ug/10ug
    Recombinant Rice Os03g0263600 protein(Os03g0263600) CSB-CF2229OFG 100ug/50ug/10ug
    Recombinant Rat Cytochrome P450 4V2(Cyp4v2) ,partial CSB-YP006456RA1 1mg
    Recombinant Human SPARC protein(SPARC) (Active) CSB-AP000301HU 10μg/50μg
    Recombinant Human Placenta growth factor protein(PGF) (Active) CSB-AP002731HU 5μg/25μg
    Recombinant Human Vascular endothelial growth factor A protein(VEGFA) (Active) CSB-AP002591HU 2ug/10μg/100ug/500ug
    Recombinant Human Fibroblast growth factor 1 protein(FGF1) (Active) CSB-AP002381HU 10μg/50μg

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