Plate set up
If the user do this assay for the first time, it is recommended to do a pre-test with two strips. A suggested plate format for pre-test is shown below. This plate format is just available for cell supernatant, tissue homogenate,urine??or serum/plasm where the recommended dilution is not provided in the manual. The user may vary the location and type of wells present as necessary for each particular experiment.
|
Std0 |
Smpl 1(1:1) |
|
Std1 |
Smpl 1(1:10) |
|
Std2 |
Smpl 1(1:100) |
|
Std3 |
Smpl 1(1:1000) |
|
Std4 |
Smpl 2(1:1) |
|
Std5 |
Smpl 2(1:10) |
|
Std6 |
Smpl 2(1:100) |
|
Std7 |
Smpl 2(1:1000) |
Std---Standard
Smpl---Sample
(1:10)---10 fold dilution
(1:100)---100 fold dilution
(1:1000)---1000 fold dilution
After the pre-test, choose a suitable dilution to go on the assay. The plate format is shown below.
|
Std0 |
Std0 |
Smpl1 |
Smpl1 |
Smpl9 |
Smpl9 |
Smpl17 |
Smpl17 |
Smpl25 |
Smpl25 |
|
Std1 |
Std1 |
Smpl2 |
Smpl2 |
Smpl10 |
Smpl10 |
Smpl18 |
Smpl18 |
Smpl26 |
Smpl26 |
|
Std2 |
Std2 |
Smpl3 |
Smpl3 |
Smpl11 |
Smpl11 |
Smpl19 |
Smpl19 |
Smpl27 |
Smpl27 |
|
Std3 |
Std3 |
Smpl4 |
Smpl4 |
Smpl12 |
Smpl12 |
Smpl20 |
Smpl20 |
Smpl28 |
Smpl28 |
|
Std4 |
Std4 |
Smpl5 |
Smpl5 |
Smpl13 |
Smpl13 |
Smpl21 |
Smpl21 |
Smpl29 |
Smpl29 |
|
Std5 |
Std5 |
Smpl6 |
Smpl6 |
Smpl14 |
Smpl14 |
Smpl22 |
Smpl22 |
Smpl30 |
Smpl30 |
|
Std6 |
Std6 |
Smpl7 |
Smpl7 |
Smpl15 |
Smpl15 |
Smpl23 |
Smpl23 |
Smpl31 |
Smpl31 |
|
Std7 |
Std7 |
Smpl8 |
Smpl8 |
Smpl16 |
Smpl16 |
Smpl24 |
Smpl24 |
Smpl32 |
Smpl32 |
|
