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Plate set up

If the user do this assay for the first time, it is recommended to do a pre-test with two strips. A suggested plate format for pre-test is shown below. This plate format is just available for cell supernatant, tissue homogenate,urine??or serum/plasm where the recommended dilution is not provided in the manual. The user may vary the location and type of wells present as necessary for each particular experiment.

Std0

Smpl 1(1:1)

Std1

Smpl 1(1:10)

Std2

Smpl 1(1:100)

Std3

Smpl 1(1:1000)

Std4

Smpl 2(1:1)

Std5

Smpl 2(1:10)

Std6

Smpl 2(1:100)

Std7

Smpl 2(1:1000)

 

 

Std---Standard

Smpl---Sample

(1:10)---10 fold dilution

(1:100)---100 fold dilution

(1:1000)---1000 fold dilution

 

 

 

After the pre-test, choose a suitable dilution to go on the assay. The plate format is shown below.

Std0

Std0

Smpl1

Smpl1

Smpl9

Smpl9

Smpl17

Smpl17

Smpl25

Smpl25

Std1

Std1

Smpl2

Smpl2

Smpl10

Smpl10

Smpl18

Smpl18

Smpl26

Smpl26

Std2

Std2

Smpl3

Smpl3

Smpl11

Smpl11

Smpl19

Smpl19

Smpl27

Smpl27

Std3

Std3

Smpl4

Smpl4

Smpl12

Smpl12

Smpl20

Smpl20

Smpl28

Smpl28

Std4

Std4

Smpl5

Smpl5

Smpl13

Smpl13

Smpl21

Smpl21

Smpl29

Smpl29

Std5

Std5

Smpl6

Smpl6

Smpl14

Smpl14

Smpl22

Smpl22

Smpl30

Smpl30

Std6

Std6

Smpl7

Smpl7

Smpl15

Smpl15

Smpl23

Smpl23

Smpl31

Smpl31

Std7

Std7

Smpl8

Smpl8

Smpl16

Smpl16

Smpl24

Smpl24

Smpl32

Smpl32

 


 

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