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cusabio

Features:
  • Risk-free: Do not charge by steps. No protein, No charge.
  • Competitive price as low as $594.
  • 39 tags meeting different demands.
  • Secondary AKTA-SEC purification to ensure high purity.
  • Ability to achieve large-scale production (10 mg, 50 mg, 100 mg, 200 mg available).
  • Post-purification services available: Desalting, aliquot, endotoxin removal, aseptic process and lyophilization.
Thousands of developed proteins are also available. Search Protein name / Target / Cat No / Alias / Abbreviations / Uniprot No. to find your desired protein in our database below.
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  • Which expression system suits your experiment most ? View the characteristics of five expression systems to help you choose one.

    The Characteristics of Five Expression Systems

    Advantages Disadvantages
    E.coli
    • 1.

      Low cost and simple culture conditions;

    • 2.

      Short protein expression period;

    • 3.

      Clear genetic background;

    • 4.

      Simple transformation operation;

    • 5.

      High rate of reproduction and expression level;

    • 6.

      A variety of carriers and fusion tags to choose;

    • 7.

      Many parameters can be altered to optimize expression;

    • 8.

      Tag can be available at both N-terminal and C-terminal with an enzyme cutting site that is easy to cut.

    • 1.

      Inefficient disulfide bond formation and poor folding lead to inclusion body formation;

    • 2.

      Poor post-translational modifications (such as glycosylation, alkylation, phosphorylation, specificity of proteolytic processing, etc.);

    • 3.

      The success rate and recovery rate of inclusion body renaturation is not very high.

    Yeast
    • 1.

      Low cost and high expression level;

    • 2.

      Self-produced Endotoxin-free;

    • 3.

      Efficient protein folding and secretory expression;

    • 4.

      Simple culture conditions and purification operation;

    • 5.

      Extensive post-translational modifications: Glycosylation, phosphorylation, acyl lipid;

    • 6.

      Can develop high density fermentation by using simple inorganic salt, large biomass;

    • 7.

      The protein is more stable than the prokaryotic expression, and it's especially suitable for the expression of eukaryotic genes and the preparation of functional expression proteins;

    • 8.

      Simple laboratory and industrial operation.

    • 1.

      Glycosylation modification is not good as mammalian cells.

    Baculovirus
    • 1.

      Good expression levels and relatively rapid growth;

    • 2.

      Glycosylation modification more like mammalian cells;

    • 3.

      More advantages over the expression of viral proteins;

    • 4.

      Relatively de-glycosylation modification (good for structure determination);

    • 5.

      Large gene volume: because the baculovirus genome is larger, so it can carry large gene fragment;

    • 6.

      High security: the baculovirus has a strict species specificity, not infected with vertebrates and plants;

    • 7.

      High expression efficiency of exogenous gene: compared with other eukaryotic expression systems, the baculovirus system can efficiently express exogenous protein from infected cells;

    • 8.

      The expression product has high activity: baculovirus will proliferation in insect host cells, thus resulting recombinant protein will be similar to mammalian cells in post-translational modification, the expression product has a strong biological activity;

    • 9.

      Easy to amplify: baculovirus can mass production of recombinant protein products with biological activity.

    • 1.

      Expensive culture media cost;

    • 2.

      Inefficient processing of pro-peptides in secretory pathway;

    • 3.

      Viral infection leads to cell lysis and potential degradation of expressed proteins;

    • 4.

      Glycosylation modification is not good as mammalian cells.

    Mammalian cells
    • 1.

      Self-produced Endotoxin-free;

    • 2.

      Efficient protein folding and secretory expression;

    • 3.

      All post-translational modifications, provide a variety of complex N glycosylation and accurate O type glycosylation and other post-translational processing functions;

    • 4.

      The expressed products are closest to the natural biological proteins in molecular structure, physical and chemical properties and biological functions.

    • 1.

      Expensive culture media cost;

    • 2.

      Complex growth requirements.

    Cell-free
    • 1.

      High expression level: we provide the original and optimized pET carrier with a series of labels, so that the membrane protein yield can reach mg grade;

    • 2.

      Protein synthesis conditions can be manipulated;

    • 3.

      The target fusion protein with 6*His is beneficial to the purification.

    • 1.

      Limited post-translational modifications;

    • 2.

      Expensive for scale-up.

    Have you already confirmed the suitable expression system ?
    No. Talk with us on the livechat or contact us through email. Together with a dedicated R&D team, our expert scientists and quality assurance team will deliver unique solutions tailored to bring you success more efficient and economically.
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