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Home > Protein Services > Eukaryotic expression service
Eukaryotic expression service
Yeast protein expression system is the most economical and efficient
eukaryotic expression system for both secretion and intracellular expression.
And the medium is relatively inexpensive for large-scale production.And the conditions for yeast cultivate are not strict,so It is ideally suitable for large-scale production of recombinant eukaryotic proteins.
The protein expressed by the mammalian cell expression system which is high-quality is very close to the natural protein.But the Low expression levels, the high cost of medium and the culture conditions relatively fastidious restrict the promotion of the use of mammalian cell expression system used to produce
high-quality protein material. as well as mammalian cell expression system, Yeast protein expression systems serve as a eukaryotic expression system integrate the advantages of the mammalian cell expression system.So the protein expressed by yeast protein expression system could be modificated such as glycosylation, acylation, Phosphorylation and so on to ensure that the protein native conformation.So It could Used to produce protein material with high added value that is very close to natural protein. The facts proved that our protein produced by Yeast protein expression system which served as raw materials for downstream preparation monoclonal antibody had good properties of the recognition of natural protein through the feedback of the customs.
our efficient seed screening work can make your order to obtain the highest yield in the shortest time
eukaryotic expression system for both secretion and intracellular expression.
And the medium is relatively inexpensive for large-scale production.And the conditions for yeast cultivate are not strict,so It is ideally suitable for large-scale production of recombinant eukaryotic proteins.
The protein expressed by the mammalian cell expression system which is high-quality is very close to the natural protein.But the Low expression levels, the high cost of medium and the culture conditions relatively fastidious restrict the promotion of the use of mammalian cell expression system used to produce
high-quality protein material. as well as mammalian cell expression system, Yeast protein expression systems serve as a eukaryotic expression system integrate the advantages of the mammalian cell expression system.So the protein expressed by yeast protein expression system could be modificated such as glycosylation, acylation, Phosphorylation and so on to ensure that the protein native conformation.So It could Used to produce protein material with high added value that is very close to natural protein. The facts proved that our protein produced by Yeast protein expression system which served as raw materials for downstream preparation monoclonal antibody had good properties of the recognition of natural protein through the feedback of the customs.
our efficient seed screening work can make your order to obtain the highest yield in the shortest time
| Yeast protein expression system economical menu service | |||
|---|---|---|---|
| Milestones | Specifications | Deliverables | Timeline |
| 1. protokaryon cloning | Genes obtain | Nucleic acid electrophoresis pictures | 2 weeks |
| PCR amplification product connected to Ppic9k modified by our company Transformate to DH5 α | |||
| Large-scale synthesis of recombinant plasmid | |||
| Linearize the recombinant plasmid | |||
| 2. Expression optimization | Electric Transformation of the appropriate yeast host strain | Nucleic acid electrophoresis pictures | 2-3 weeks |
| PCR verification of 10 strains randomly selected from transformation plate | |||
| 3. Protein expression and purification | randomly select a PCR verification positive strain for 1 L large-scale culture | Expressed supernatant SDS-PAGE |
1-2 weeks |
| The dispose of the supernatant and One-step purification | Purified product SDS-PAGE and western blot | ||
| Purified product SDS-PAGE and western blot | 1L supernatant Purified product | ||
| Yeast protein expression system service standard menu | |||
|---|---|---|---|
| Milestones | Specifications | Deliverables | Timeline |
| 1. protokaryon cloning | Genes obtain | Nucleic acid electrophoresis pictures | 2 weeks |
| PCR amplification product connected to Ppic9k modified by our company，Transformate to DH5 α | |||
| Linearize the recombinant plasmid | |||
| Linearize the recombinant plasmid | |||
| 2. Expression optimization | Electric Transformation of the appropriate yeast host strain | Nucleic acid electrophoresis pictures | 5-6 weeks |
| The poly copies screening via genetic G418 | |||
| PCR identification of multi-copy cloning | |||
| Select more than 40 multi-copies strains for small-scale expression | Purified product SDS-PAGE and western blot | ||
| 3. Protein expression and purification | Select the best strain for large-scale expression according to the customer request |
Expressed supernatant SDS-PAGE < |
|
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Manual: Eukaryotic expression