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Yeast Expression Service

Yeast protein expression system is the most economical and efficient
eukaryotic expression system for both secretion and intracellular expression.
And the medium is relatively inexpensive for large-scale production.And the conditions for yeast cultivate are not strict,so It is ideally suitable for large-scale production of recombinant eukaryotic proteins.
The protein expressed by the mammalian cell expression system  which is    high-quality is very close to the natural protein.But the Low expression levels, the high cost of medium and the culture conditions relatively fastidious restrict the promotion of the use of mammalian cell expression system used to produce             
high-quality protein material. as well as mammalian cell expression system, Yeast protein expression systems serve as a eukaryotic expression system integrate the advantages of the mammalian cell expression system.So the protein expressed by yeast protein expression system could be modificated such as glycosylation, acylation, Phosphorylation and so on to ensure that the protein  native conformation.So It could Used to produce protein material with high added value that is very close to  natural protein. The facts proved that our protein produced by Yeast protein expression system which served as raw materials for downstream preparation monoclonal antibody had good properties of the recognition of natural protein through the feedback of the customs.
our efficient seed screening work can make your order to obtain the highest yield in the shortest time
 
Yeast protein expression system economical menu service
Milestones Specifications Deliverables Timeline
1. protokaryon cloning Genes obtain Nucleic acid electrophoresis pictures 2 weeks
PCR amplification product connected to Ppic9k modified by our company Transformate to DH5 α
Large-scale synthesis of recombinant plasmid
Linearize the recombinant plasmid
2. Expression optimization Electric Transformation of the appropriate yeast host strain Nucleic acid electrophoresis pictures 2-3 weeks
PCR verification of 10 strains randomly selected from transformation plate
3. Protein expression and purification randomly select a PCR verification positive strain for 1 L large-scale culture Expressed supernatant
SDS-PAGE
1-2 weeks
The dispose of the supernatant and One-step purification Purified product SDS-PAGE and western blot
Purified product SDS-PAGE and western blot 1L supernatant Purified product

 

 

 

 

 

 

 

 

 

 

 

Yeast protein expression system service standard menu
Milestones Specifications Deliverables Timeline
1. protokaryon cloning Genes obtain Nucleic acid electrophoresis pictures 2 weeks
PCR amplification product connected to Ppic9k modified by our company,Transformate to DH5 α
Linearize the recombinant plasmid
Linearize the recombinant plasmid
2. Expression optimization Electric Transformation of the appropriate yeast host strain Nucleic acid electrophoresis pictures 5-6 weeks
The poly copies screening via genetic G418
PCR identification of multi-copy cloning
Select more than 40 multi-copies strains for small-scale expression Purified product SDS-PAGE and western blot
3. Protein expression and purification Select the best strain for large-scale expression according to the customer request

Expressed supernatant SDS-PAGE <

 

 

 

 

 

 

 

 

 

 

 

 

 

 

Order Information

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Tele:+86-27-87582341

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Manual: Eukaryotic expression