ipaD Antibody

Datasheet

Code	CSB-PA325954ZA01SZB
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: IPAD antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 52.7 kDa Observed band size: 56 kDa
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Target Background

Product Details

Uniprot No.

P18013

Target Names

ipaD

Alternative Names

Invasin IpaD (36 kDa membrane antigen), ipaD

Species Reactivity

Shigella flexneri

Immunogen

Recombinant Shigella flexneri Invasin IpaD (IpaD) (1-332aa)

Immunogen Species

Shigella flexneri

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The polyclonal antibody against ipaD can be generated by immunizing a rabbit with the recombinant invasin ipaD protein (1-332aa) from Shigella flexneri. Once collected from the rabbit serum, the ipaD antibody is further purified using protein G. This specific ipaD antibody is ideal for detecting the ipaD protein in Shigella flexneri through ELISA and WB applications.

The ipaD protein, a component of the type III secretion system (T3SS) of Shigella flexneri, plays a crucial role in the pathogenesis of Shigella flexneri by promoting invasion of the host intestinal epithelial cells. IpaD acts as a gatekeeper protein, controlling the secretion and translocation of other effector proteins through the T3SS. It also helps in the formation of a pore complex that allows the bacteria to enter the host cell.

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Target Background

Function

Required for bacterial invasion of host cells. Controls IpaB and IpaC secretion, and the efficiency with which they are physically inserted into target cell membranes. These proteins are exported via TTSS to form a pore in the host membrane that allows the translocation of the other effectors into the host cytoplasm. Along with IpaB, is essential for both blocking secretion through the Mxi/Spa translocon in the absence of a secretion-inducing signal, and for controlling the level of secretion in the presence of this signal.

Gene References into Functions

IpaD, the putative needle-tip protein of the S. flexneri type III secretion system, has been crystallized; in-drop proteolysis resulted in the production of several new crystal forms. PMID: 16946465
IpaD forms part of the structure at the needle tip in Shigella flexner and antibodiesblock entry of bacteria into epithelial cells. PMID: 17110044

Subcellular Location

Secreted. Note=Secreted via the type III secretion system (TTSS). Localizes to the tip of the external secretion needle that is part of the TTSS apparatus.

Protein Families

Invasin protein D family

Database Links

KEGG: sfl:CP0126

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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ipaD Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background