Product Details

Target Name

lactalbumin, alpha-

Alternative Names

LALBA ELISA Kit; ALACTA ELISA Kit; Alpha-lactalbumin ELISA Kit; Lactose synthase B protein ELISA Kit; allergen Bos d 4 ELISA Kit

Abbreviation

LALBA

Uniprot No.

P00711

Species

Bos taurus (Bovine)

Sample Types

serum, plasma

Detection Range

0.054 ng/ml - 40 ng/ml

Sensitivity

0.054 ng/ml

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Competitive

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of bovine α-La in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	80-90
1:2	Average %	93
	Range %	89-97
1:4	Average %	95
	Range %	90-100
1:8	Average %	90
	Range %	85-97

Recovery

The recovery of bovine α-La spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	103	99-108

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml	OD1	OD2	Average
10	0.186	0.180	0.183
5	0.302	0.291	0.2965
2.5	0.397	0.389	0.393
1.25	0.523	0.495	0.509
0.625	0.724	0.747	0.7355
0.312	1.013	1.024	1.0185
0.156	1.322	1.293	1.3075
0	2.163	2.052	2.1075

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Bovine LALBA ELISA Kit was designed for the quantitative measurement of Bovine LALBA protein in serum, plasma. It is a Competitive ELISA kit, its detection range is 0.054 ng/ml - 40 ng/ml and the sensitivity is 0.054 ng/ml.

Target Background

Function
(From Uniprot)

Regulatory subunit of lactose synthase, changes the substrate specificity of galactosyltransferase in the mammary gland making glucose a good acceptor substrate for this enzyme. This enables LS to synthesize lactose, the major carbohydrate component of milk. In other tissues, galactosyltransferase transfers galactose onto the N-acetylglucosamine of the oligosaccharide chains in glycoproteins.

Gene References into Functions

This study shows, for the first time, that alpha-lactalbumin isolated in a rare 28kDa dimeric form induces cell death, while 14kDa monomeric alpha-lactalbumin is inactive. PMID: 28193539
Macromolecular crowding reduces the calcium binding affinity of holo-lactalbumin alpha resulting in the formation of apo-lactalbumin alpha (the calcium-depleted form of holo-LA) leading to aggregate formation. PMID: 25437004
Data suggest that stabilization of apo-alpha-lactalbumin by mono- and oligo-saccharides can be accounted for by simplified statistical-thermodynamic model considering volume exclusion deriving from steric repulsion between protein and saccharides. PMID: 26000826
These findings suggest that the SNP alpha-LA2516 in the alpha-LA gene likely does not have potential as a molecular marker for milk production traits in Chinese Holstein cows. PMID: 24065678
alphaLA forms a misfolded disulfide bond shuffled isomer, X-alphaLA which represents a different low energy fold for the protein; binding calcium plays an important role in both maintaining the native structure of alphaLA and providing a mechanism for distinguishing between folded and misfolded species. PMID: 22189830
Structural features of the alphaB-crystallin oligomer when complexed with target proteins under mild stress conditions, i.e., reduction of alpha-lactalbumin at 37 degrees C and malate dehydrogenase when heated at 42 degrees C, were investigated. PMID: 21152271
the organization and dynamics of the functionally important tryptophan residues of BLA in native, molten globule and denatured states utilizing the wavelength-selective fluorescence approach were explored. PMID: 20346348
The complexes formed by partially folded alpha-lactalbumin with oleic acid display selective apoptotic activity against tumor cells. PMID: 19968717
a functional Ca(2+)-binding site is not required for conversion of alpha-lactalbumin to the active complex or to cause cell death PMID: 14627739
Negatively charged alpha-lactalbumin (LA) interaction with basic histone stabilizes apo-alpha-LA and destabilizes the Ca2+-bound protein due to compensation for excess negative charge of alpha-LA's Ca2+-binding loop by positively charged histone residues. PMID: 15134431
interaction of alphaB-cyrstallin with, and promoted unfolding of, reduced alpha-lactalbumin, but showed limited chaperone activity for other target proteins PMID: 15670152
Data indicate that deletion of the beta-subdomain in alpha-lactalbumin does not alter the ability of the protein to assemble into well-ordered fibrils, implying that this chain region is not essential for the amyloid formation. PMID: 15853802
Fold and topology of alpha-lactalbumin may be formed from degenerate groups of side chains. PMID: 15956205
residual long-range interactions may act as nucleation sites for protein folding, while this property of residual structure is replaced by the calcium-binding site between the domains in alpha-lactalbumin. PMID: 16731974
characterized the acid induced molten globule state (A-state) of alpha-LA both qualitatively and quantitatively. PMID: 16741984
Results describe the reaction mechanism and formation of CIDNP (chemically induced dynamic nuclear polarization) in the photoreactions of the dye 2,2'-dipyridyl with non-native states of bovine and human alpha-lactalbumins in aqueous solution. PMID: 16851644
interaction between calcium-depleted alpha-lactalbumin and lysozyme leads to the formation of different supramolecular structures depending on the temperature PMID: 17260954
stopped-flow CD, fluorescence, and time-resolved NMR studies that the Ca(2+)-induced refolding of bovine alpha-lactalbumin (BLA) at constant denaturant concentration (4 M urea) exhibits triple-exponential kinetics. PMID: 18377934
Allelic frequencies and RFLP patterns of alpha-lactalbumin gene vary between two species of cattle. PMID: 18607792
Disulfide bond shuffling in bovine alpha-lactalbumin: MD simulation confirms experiment PMID: 18942855
Serotonin suppressed alpha-lactalbumin mRNA expression in lactogen-treated primary bovine mammary epithelial cell cultures. PMID: 19654143

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Subcellular Location

Secreted.

Protein Families

Glycosyl hydrolase 22 family

Tissue Specificity

Mammary gland specific. Secreted in milk.

Database Links

KEGG: bta:281894

STRING: 9913.ENSBTAP00000007701

UniGene: Bt.87412

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Code	CSB-E13200B
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Bovine α Lactalbumin,α-La ELISA Kit

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