Bovine A disintegrin and metalloproteinase with thrombospondin motifs 5(ADAMTS5) ELISA kit

Code CSB-EL001312BO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9TT92
Lead Time 7-14 working days
Abbreviation ADAMTS5
Target Name ADAM metallopeptidase with thrombospondin type 1 motif, 5
Alias ADAMTS11, ADMP-2, FLJ36738, a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 5 (aggrecanase-2)|aggrecanase-2
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The enzyme encoded by this gene contains two C-terminal TS motifs and functions as aggrecanase to cleave aggrecan, a major proteoglycan of cartilage.
HGNC 221
RGD 735196
MGI 1346321
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of bovine ADAMTS5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 87
Range % 83-92
1:2 Average % 102
Range % 97-107
1:4 Average % 91
Range % 85-97
1:8 Average % 94
Range % 91-98
The recovery of bovine ADAMTS5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 89-99
EDTA plasma (n=4) 98 93-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
40 2.541 2.675 2.608 2.496
20 2.000 2.134 2.067 1.955
10 1.341 1.386 1.364 1.252
5 0.758 0.766 0.762 0.650
2.5 0.416 0.424 0.420 0.308
1.25 0.285 0.273 0.279 0.167
0.625 0.177 0.174 0.176 0.064
0 0.111 0.112 0.112  
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Function Cleaves aggrecan, a cartilage proteoglycan, at the '392-Glu-|-Ala-393' site, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Subcellular Location Secreted, extracellular space, extracellular matrix
Database Links

STRING: 9913.ENSBTAP00000000849

UniGene: Bt.13108

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