Bovine Beta-defensin 119(DEFB119) ELISA kit

Unavailable
Code CSB-EL006690BO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q32P86
Lead Time 7-14 working days
Abbreviation DEFB119
Target Name defensin, beta 119
Alias DEFB-19, DEFB-20, DEFB120, ESC42-RELA, ESC42-RELB, MGC71893, defensin, beta 120|defensin, beta 19|defensin, beta 20
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 18099
RGD 1559541
MGI 2385955
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine DEFB119 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 92-100
1:2 Average % 90
Range % 82-97
1:4 Average % 103
Range % 99-110
1:8 Average % 87
Range % 82-98
Recovery
The recovery of bovine DEFB119 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-97
EDTA plasma (n=4) 108 105-114
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
10 1.898 1.902 1.900 1.795
5 1.692 1.633 1.663 1.558
2.5 1.314 1.363 1.339 1.234
1.25 1.001 1.021 1.011 0.906
0.625 0.660 0.686 0.673 0.568
0.312 0.448 0.466 0.457 0.352
0.156 0.281 0.275 0.278 0.173
0 0.107 0.102 0.105  
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Function Has antibacterial activity.
Subcellular Location Secreted
Protein Families Beta-defensin family
Database Links

KEGG: bta:767957

STRING: 9913.ENSBTAP00000004362

UniGene: Bt.46959

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