Bovine C-C motif chemokine 5(CCL5) ELISA kit

Code CSB-EL004800BO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name chemokine (C-C motif) ligand 5
Alternative Names CCL5 ELISA kit; SCYA5C-C motif chemokine 5 ELISA kit; SIS-delta ELISA kit; Small-inducible cytokine A5 ELISA kit; T-cell-specific protein RANTES ELISA kit
Abbreviation CCL5
Uniprot No. O97919
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 4.7 ng/mL-300 ng/mL
Sensitivity 1.17 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of bovine CCL5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 97
Range % 87-101
1:2 Average % 98
Range % 92-101
1:4 Average % 104
Range % 93-108
1:8 Average % 99
Range % 93-102
The recovery of bovine CCL5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 101 93-105
EDTA plasma (n=4) 95 84-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
300 2.564 2.486 2.525 2.364
150 1.967 1.892 1.930 1.769
75 1.319 1.367 1.343 1.182
37.5 0.890 0.865 0.878 0.717
18.75 0.508 0.521 0.515 0.354
9.4 0.359 0.347 0.353 0.192
4.7 0.262 0.258 0.260 0.099
0 0.159 0.163 0.161  
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. May activate several chemokine receptors including CCR1, CCR3, CCR4 and CCR5. May also be an agonist of the G protein-coupled receptor GPR75. Together with GPR75, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. By activating GPR75 may also play a role in insulin secretion by islet cells.
Gene References into Functions
  1. CCL5 but not CCL2 mainly attract bovine classical monocytes and promote their differentiation into LPS-hypo-responsive macrophages. PMID: 25064684
  2. decrease in sensitivity of HIV variants to RANTES neutralization during the course of progressive infection, but not during follow-up of long term survivors; data suggest a role for RANTES neutralization sensitivity of HIV-1 in AIDS pathogenesis PMID: 12964118
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Database Links

KEGG: bta:327712

STRING: 9913.ENSBTAP00000009463

UniGene: Bt.552


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1