Bovine Calcitonin(CALCA/CALC) ELISA kit

Code CSB-E17374B
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P01260
Lead Time 3-5 working days
Abbreviation CALCA/CALC
Target Name Calcitonin(CALCA/CALC)
Alias CALC1, CGRP, CGRP-I, CGRP1, CT, KC, MGC126648, calcitonin 1|calcitonin gene-related peptide 1|calcitonin/calcitonin-related polypeptide, alpha|katacalcin
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine CALCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %100
Range %94-105
1:2Average %86
Range %81-90
1:4Average %103
Range %96-107
1:8Average %98
Range %89-103
Recovery
The recovery of bovine CALCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9083-94
EDTA plasma (n=4)8884-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
4000.144 0.138 0.141
2000.286 0.276 0.281
1000.463 0.455 0.459
500.857 0.840 0.849
251.821 1.765 1.793
References
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Function Causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones.
Subcellular Location Secreted
Protein Families Calcitonin family
Database Links

STRING: 9913.ENSBTAP00000053947

UniGene: Bt.14302

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