Bovine Cardiac Troponin T(cTn-T) ELISA kit

Instructions
Code CSB-E13646B
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Cardiac Troponin T(cTn-T)
Alternative Names TNNC1 ELISA Kit; TNNC ELISA Kit; Troponin C ELISA Kit; slow skeletal and cardiac muscles ELISA Kit; TN-C ELISA Kit
Abbreviation cTn-T
Uniprot No. P63315
Species Bos taurus (Bovine)
Sample Types serum, plasma
Detection Range 187.5 pg/mL- 3000 pg/mL
Sensitivity 187.5 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine cTn-T in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %88-98
1:2Average %95
Range %91-99
1:4Average %101
Range %98-105
1:8Average %88
Range %82-94
Recovery
The recovery of bovine cTn-T spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9390-96
EDTA plasma (n=4)10298-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
30000.154 0.155 0.155
15000.278 0.284 0.281
7500.563 0.542 0.553
3751.145 1.223 1.184
187.52.345 2.234 2.290
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Troponin is the central regulatory protein of striated muscle contraction. Tn consists of three components
Gene References into Functions
  1. These results demonstrate that the L29Q mutation enhances the Ca(2+)-binding characteristics of cTnC and that when incorporated into cardiac myocytes, this mutant alters myocyte contractility. PMID: 18285522
Protein Families Troponin C family
Database Links

KEGG: bta:509486

STRING: 9913.ENSBTAP00000055780

UniGene: Bt.49083

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