Bovine Complement 4(C4) ELISA kit

Code CSB-EL003949BO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Complement 4(C4)
Abbreviation C4
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.156 μg/mL-10 μg/mL
Sensitivity 0.078 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Competitive
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of bovine complement 4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:100 Average % 95
Range % 85-101
1:200 Average % 97
Range % 90-105
1:400 Average % 97
Range % 86-110
1:800 Average % 95
Range % 86-99
The recovery of bovine complement 4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 89-100
EDTA plasma (n=4) 96 87-102
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average
10 0.245 0.249 0.247
5 0.386 0.402 0.394
2.5 0.538 0.552 0.545
1.25 0.805 0.801 0.803
0.625 0.985 0.997 0.991
0.312 1.248 1.262 1.255
0.156 1.577 1.593 1.585
0 2.399 2.428 2.414
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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