Bovine Corticoliberin(CRH) ELISA kit

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Code CSB-EL005963BO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q95MI6
Lead Time 7-14 working days
Abbreviation CRH
Target Name corticotropin releasing hormone
Alias CRF, corticotropin-releasing factor
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 25 pg/mL-1000 pg/mL
Sensitivity 25 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Target Details Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin dificiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta CRH is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, CRH may act as a trigger for parturition.
HGNC 2355
RGD 620505
MGI 88496
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine CRH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-92
1:2 Average % 90
Range % 86-100
1:4 Average % 95
Range % 92-106
1:8 Average % 93
Range % 86-99
Recovery
The recovery of bovine CRH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 90 85-97
EDTA plasma (n=4) 89 85-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average  
1000 0.152 0.156 0.154  
500 0.357 0.362 0.360  
230 0.568 0.570 0.569  
75 0.869 0.875 0.872  
25 1.554 1.567 1.561  
0 2.376 2.381 2.379  
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Function Hormone regulating the release of corticotropin from pituitary gland (By similarity). Induces NLRP6 in intestinal epithelial cells, hence may influence gut microbiota profile (By similarity).
Subcellular Location Secreted
Protein Families Sauvagine/corticotropin-releasing factor/urotensin I family
Tissue Specificity Produced by the hypothalamus.
Database Links

KEGG: bta:280755

STRING: 9913.ENSBTAP00000044298

UniGene: Bt.37342

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