Bovine Endothelin-converting enzyme 1(ECE1) ELISA kit

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Code CSB-EL007371BO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P42891
Lead Time 7-14 working days
Abbreviation ECE1
Target Name endothelin converting enzyme 1
Alias RP3-329E20.1, ECE,
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
HGNC 3146
RGD 620293
MGI 1101357
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine ECE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 90
Range % 85-95
1:2 Average % 95
Range % 91-100
1:4 Average % 94
Range % 86-98
1:8 Average % 99
Range % 92-103
Recovery
The recovery of bovine ECE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 96 89-101
EDTA plasma (n=4) 102 96-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 1.814 1.896 1.855 1.755
10 1.438 1.407 1.423 1.323
5 0.995 1.028 1.012 0.912
2.5 0.649 0.641 0.645 0.545
1.25 0.432 0.424 0.428 0.328
0.625 0.329 0.319 0.324 0.224
0.312 0.184 0.178 0.181 0.081
0 0.102 0.098 0.100  
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Function Converts big endothelin-1 to endothelin-1.
Subcellular Location Cell membrane, Single-pass type II membrane protein
Protein Families Peptidase M13 family
Database Links

STRING: 9913.ENSBTAP00000055669

UniGene: Bt.4378

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