Bovine Heat shock protein beta-1(HSPB1) ELISA kit

Code CSB-EL010833BO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q3T149
Lead Time 5-7 working days
Abbreviation HSPB1
Protein Biological Process 1 Neurobiology
Target Name heat shock 27kDa protein 1
Alias CMT2F, DKFZp586P1322, HMN2B, HS.76067, HSP27, HSP28, Hsp25, SRP27, estrogen-regulated 24 kDa protein|heat shock 27kD protein 1|heat shock protein beta-1|stress-responsive protein 27
Species Bos taurus (Bovine)
Protein Biological Process 3 Stress response
Sample Types serum, plasma, tissue homogenates
Detection Range 0.235 ng/mL-15 ng/mL
Sensitivity 0.058 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is induced by environmental stress and developmental changes. The encoded protein is involved in stress resistance and actin organization and translocates from the cytoplasm to the nucleus upon stress induction. Defects in this gene are a cause of Charcot-Marie-Tooth disease type 2F (CMT2F) and distal hereditary motor neuropathy (dHMN).
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %90
Range %87-92
1:2Average %101
Range %97-105
1:4Average %93
Range %88-97
1:8Average %91
Range %88-95
Recovery
The recovery of bovine HSPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9087-93
EDTA plasma (n=4)9086-94
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
152.679 2.498 2.589 2.481
7.52.121 2.246 2.184 2.076
3.751.589 1.707 1.648 1.540
1.871.123 1.054 1.089 0.981
0.940.702 0.680 0.691 0.583
0.470.422 0.444 0.433 0.325
0.2350.274 0.256 0.265 0.157
00.107 0.109 0.108
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Function Small heat shock protein which functions as a molecular chaperone probably maintaining denatured proteins in a folding-competent state. Plays a role in stress resistance and actin organization. Through its molecular chaperone activity may regulate numerous biological processes including the phosphorylation and the axonal transport of neurofilament proteins.
Subcellular Location Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, spindle
Protein Families Small heat shock protein (HSP20) family
Database Links

STRING: 9913.ENSBTAP00000015883

UniGene: Bt.4415

Pathway MAPK signaling pathway
VEGF signaling pathway

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