Bovine Hydroxymethylglutaryl-CoA lyase, mitochondrial(HMGCL) ELISA kit

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Code CSB-EL010563BO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q29448
Lead Time 7-14 working days
Abbreviation HMGCL
Target Name 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase
Alias HL, 3-hydroxy-3-methylglutaryl CoA lyase|3-hydroxy-3-methylglutaryl-CoA lyase|HMG-CoA lyase|hydroxymethylglutaricaciduria
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 23.5 pg/mL-1500 pg/mL
Sensitivity 5.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
HGNC 5005
RGD 620554
MGI 96158
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine HMGCL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 81-92
1:2 Average % 102
Range % 97-107
1:4 Average % 92
Range % 85-96
1:8 Average % 103
Range % 96-107
Recovery
The recovery of bovine HMGCL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 94 89-98
EDTA plasma (n=4) 102 97-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
1500 2.245 2.312 2.279 2.164
750 1.803 1.889 1.846 1.731
375 1.386 1.424 1.405 1.290
187.5 0.988 0.976 0.982 0.867
94 0.631 0.643 0.637 0.522
47 0.418 0.424 0.421 0.306
23.5 0.255 0.259 0.257 0.142
0 0.114 0.115 0.115  
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Function Key enzyme in ketogenesis (ketone body formation). Terminal step in leucine catabolism. Ketone bodies (beta-hydroxybutyrate, acetoacetate and acetone) are essential as an alternative source of energy to glucose, as lipid precursors and as regulators of metabolism (By similarity).
Subcellular Location Mitochondrion matrix, Peroxisome
Protein Families HMG-CoA lyase family
Database Links

KEGG: bta:317658

STRING: 9913.ENSBTAP00000029103

UniGene: Bt.9446

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