Bovine Inhibin alpha chain(INHA) ELISA kit

Instructions
Code CSB-EL011718BO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name inhibin, alpha
Alternative Names INHA ELISA Kit; Inhibin alpha chain ELISA Kit
Abbreviation INHA
Uniprot No. P07994
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 20 pg/mL-4000 pg/mL
Sensitivity 10 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine INHA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 95
Range % 90-101
1:2 Average % 92
Range % 89-99
1:4 Average % 90
Range % 87-100
1:8 Average % 92
Range % 85-99
Recovery
The recovery of bovine INHA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 102 95-110
EDTA plasma (n=4) 92 87-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average
0 2.652 2.631 2.642
20 1.359 1.343 1.351
80 0.924 0.960 0.942
320 0.476 0.495 0.486
1000 0.353 0.367 0.360
4000 0.162 0.159 0.161
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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Target Data

Function Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins.
Gene References into Functions
  1. The effects of castration and other surgical intervention on the blood levels of anti-Muellerian hormone, inhibin A, gonadotropins, and gonadotropin receptors in bull calves are reported. PMID: 27693012
  2. The expression of the components of the activin-inhibin-follistatin system is altered in bovine cystic ovarian disease. PMID: 25001504
  3. INHA gene can be used as a predictor for superovulation in Chinese Holstein cows. PMID: 20238172
  4. Inhibin has a negative autocrine or paracrine effect on the in vitro capacity of granulosa cells isolated from dominant or subordinate follicles to produce estradiol. PMID: 12697695
  5. overexpression of inhibin alpha (1-32) fragment regulated granulosa cell development and effects on subsequent oocyte maturation were both time- and stage-dependent PMID: 18456314

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Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

KEGG: bta:281254

STRING: 9913.ENSBTAP00000013154

UniGene: Bt.36723

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