Bovine Integrin beta-5(ITGB5) ELISA kit

Unavailable
Code CSB-EL011889BO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P80747
Lead Time 5-7 working days
Abbreviation ITGB5
Protein Biological Process 1 Cell Adhesion
Target Name integrin, beta 5
Alias FLJ26658,
Species Bos taurus (Bovine)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 39 pg/mL-2500 pg/mL
Sensitivity 9.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 6160
RGD 628869
MGI 96614
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine ITGB5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 85-102
1:2 Average % 94
Range % 90-100
1:4 Average % 95
Range % 88-101
1:8 Average % 96
Range % 87-99
Recovery
The recovery of bovine ITGB5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 92 87-99
EDTA plasma (n=4) 97 90-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2500 2.054 2.087 2.071 1.854
1250 1.685 1.721 1.703 1.486
625 1.313 1.327 1.320 1.103
312.5 0.952 0.988 0.970 0.753
156.25 0.684 0.649 0.667 0.450
78.12 0.425 0.436 0.431 0.214
39 0.321 0.315 0.318 0.101
0 0.218 0.215 0.217  
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Function Integrin alpha-V/beta-5 (ITGAV
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Integrin beta chain family
Database Links

STRING: 9913.ENSBTAP00000018278

UniGene: Bt.9502

Pathway PI3K-Akt signaling pathway
Focal adhesion
Regulation of actin cytoskeleton

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