Bovine Intercellular adhesion molecule 1(ICAM1) ELISA kit

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Code CSB-EL010949BO
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q95132
Lead Time 5-7 working days
Abbreviation ICAM1
Protein Biological Process 1 Cell Adhesion
Target Name intercellular adhesion molecule 1
Alias BB2, CD54, P3.58, cell surface glycoprotein P3.58|human rhinovirus receptor|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
Species Bos taurus (Bovine)
Protein Biological Process 3 Cell adhesion
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 0.625 ng/mL-40 ng/mL
Sensitivity 0.156 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Competitive
Target Details This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a / CD18, or CD11b / CD18 and is also exploited by Rhinovirus as a receptor.
HGNC 5344
RGD 2857
MGI 96392
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine ICAM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:5 Average % 92
Range % 87-96
1:10 Average % 95
Range % 91-99
1:20 Average % 104
Range % 98-114
1:40 Average % 88
Range % 84-92
Recovery
The recovery of bovine ICAM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 93 88-97
EDTA plasma (n=4) 104 99-108
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average
40 0.097 0.099 0.098
20 0.154 0.160 0.157
10 0.236 0.241 0.239
5 0.355 0.365 0.360
2.5 0.532 0.544 0.538
1.25 0.674 0.713 0.694
0.625 0.892 0.954 0.923
0 1.662 1.735 1.699
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Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily, ICAM family
Database Links

KEGG: bta:281839

STRING: 9913.ENSBTAP00000013608

UniGene: Bt.5372

Pathway Lipids and Inflammation in Atherogenesis
NF-kappa B signaling pathway
TNF signaling pathway
Leukocyte transendothelial migration
Natural killer cell mediated cytotoxicity
AGE-RAGE signaling pathway in diabetic complications

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