Bovine Interferon omega-1(IFNW1) ELISA kit

Code CSB-EL011061BO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P07352
Lead Time 5-7 working days
Abbreviation IFNW1
Protein Biological Process 1 Immunity
Target Name interferon, omega 1
Alias , IFN-omega 1, interferon omega-1|interferon omega-1
Species Bos taurus (Bovine)
Protein Biological Process 3 Antiviral defense
Sample Types serum, plasma, tissue homogenates
Detection Range 62.5 pg/mL-4000 pg/mL
Sensitivity 15.6 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IFNW1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 83
Range % 80-86
1:2 Average % 95
Range % 91-99
1:4 Average % 88
Range % 84-92
1:8 Average % 89
Range % 85-92
Recovery
The recovery of bovine IFNW1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 93-100
EDTA plasma (n=4) 93 88-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
4000 2.462 2.587 2.525 2.423
2000 2.009 1.998 2.004 1.902
1000 1.561 1.598 1.580 1.478
500 1.038 1.068 1.053 0.951
250 0.650 0.678 0.664 0.562
125 0.362 0.354 0.358 0.256
62.5 0.210 0.201 0.206 0.104
0 0.100 0.103 0.102  
References
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Subcellular Location Secreted
Protein Families Alpha/beta interferon family
Database Links

KEGG: bta:281847

STRING: 9913.ENSBTAP00000055699

UniGene: Bt.45039

Pathway Jak-STAT signaling pathway

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