Bovine Tyrosinase(TYR) ELISA kit

Instructions
Code CSB-EL025394BO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name tyrosinase (oculocutaneous albinism IA)
Alternative Names TYR ELISA Kit; Tyrosinase ELISA Kit; EC 1.14.18.1 ELISA Kit; Monophenol monooxygenase ELISA Kit
Abbreviation TYR
Uniprot No. Q8MIU0
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Metabolism
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine TYR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %86-94
1:2Average %96
Range %89-101
1:4Average %95
Range %87-102
1:8Average %93
Range %88-99
Recovery
The recovery of bovine TYR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9792-102
EDTA plasma (n=4)9588-103
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.168 2.193 2.181 2.044
10001.642 1.668 1.655 1.518
5001.152 1.171 1.162 1.025
2500.837 0.803 0.820 0.683
1250.492 0.460 0.476 0.339
62.50.341 0.343 0.342 0.205
31.250.245 0.238 0.242 0.105
00.139 0.135 0.137  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the initial and rate limiting step in the cascade of reactions leading to melanin production from tyrosine. In addition to hydroxylating tyrosine to DOPA (3,4-dihydroxyphenylalanine), also catalyzes the oxidation of DOPA to DOPA-quinone, and possibly the oxidation of DHI (5,6-dihydroxyindole) to indole-5,6 quinone.
Involvement in disease Defects in TYR are the cause of a form of albinism in Braunvieh calf.
Subcellular Location Melanosome membrane, Single-pass type I membrane protein, Melanosome
Protein Families Tyrosinase family
Database Links

KEGG: bta:280951

STRING: 9913.ENSBTAP00000015679

UniGene: Bt.9028

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