Bovine chorionic somatomammotropin hormone (CSH1) ELISA kit

Instructions
Code CSB-EL006053BO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name chorionic somatomammotropin hormone 1 (placental lactogen)
Alternative Names CSH1 ELISA kit; PL1Chorionic somatomammotropin hormone 1 ELISA kit; BPLP-I ELISA kit; Placental lactogen I ELISA kit
Abbreviation CSH1
Uniprot No. P09611
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 80 ng/mL-2000 ng/mL
Sensitivity 50 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine CSH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 83-109  
1:2 Average % 94  
Range % 85-103  
1:4 Average % 90  
Range % 86-102  
1:8 Average % 92  
Range % 87-101  
Recovery
The recovery of bovine CSH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 86-104  
EDTA plasma (n=4) 97 85-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
2000 0.133 0.140 0.137    
800 0.229 0.237 0.233    
480 0.342 0.362 0.352    
200 0.618 0.596 0.607    
80 1.296 1.254 1.275    
0 2.703 2.741 2.722    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

STRING: 9913.ENSBTAP00000035394

UniGene: Bt.430

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