Bovine chorionic somatomammotropin hormone (CSH1) ELISA kit

Code CSB-EL006053BO
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P09611
Lead Time 3-5 working days
Abbreviation CSH1
Target Name chorionic somatomammotropin hormone 1 (placental lactogen)
Alias CSA, CSMT, FLJ75407, PL, CS-1|choriomammotropin|chorionic somatomammotropin A|chorionic somatomammotropin hormone 1|hCS-A|placental lactogen
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 80 ng/mL-2000 ng/mL
Sensitivity 50 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Target Details This protein is a member of the somatotropin/prolactin family of hormones and plays an important role in growth control. The gene is located at the growth hormone locus on chromosome 17 along with four other related genes in the same transcriptional orientation; an arrangement which is thought to have evolved by a series of gene duplications. Although the five genes share a remarkably high degree of sequence identity, they are expressed selectively in different tissues. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed mainly in the placenta and utilizes multiple transcription initiation sites. Expression of the identical mature proteins for chorionic somatomammotropin hormones 1 and 2 is upregulated during development, although the ratio of 1 to 2 increases by term. Mutations in this gene result in placental lactogen deficiency and Silver-Russell syndrome.
HGNC 2440
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine CSH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 83-109  
1:2 Average % 94  
Range % 85-103  
1:4 Average % 90  
Range % 86-102  
1:8 Average % 92  
Range % 87-101  
Recovery
The recovery of bovine CSH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 94 86-104  
EDTA plasma (n=4) 97 85-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
2000 0.133 0.140 0.137    
800 0.229 0.237 0.233    
480 0.342 0.362 0.352    
200 0.618 0.596 0.607    
80 1.296 1.254 1.275    
0 2.703 2.741 2.722    
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Subcellular Location Secreted
Protein Families Somatotropin/prolactin family
Database Links

STRING: 9913.ENSBTAP00000035394

UniGene: Bt.430

Pathway Jak-STAT signaling pathway
PI3K-Akt signaling pathway

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