Bovine hepcidin 25(Hepc25)ELISA kit

Code CSB-EQ028043BO
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Product Type ELISA kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation Hepc25
Target Name hepcidin 25(Hepc25)
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 20 ng/mL-640 ng/mL
Sensitivity 10 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hepc25 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %87-96
1:2Average %97
Range %92-101
1:4Average %86
Range %82-90
1:8Average %100
Range %96-108
Recovery
The recovery of bovine Hepc25 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9085-94
EDTA plasma (n=4)9590-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
6402.643 2.552 2.598 2.527
3201.639 1.681 1.660 1.589
1400.891 0.857 0.874 0.803
600.487 0.495 0.491 0.420
200.204 0.198 0.201 0.130
00.072 0.070 0.071
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