Bovine substance P (SP) ELISA kit

Code CSB-EQ027765BO
See More Details 24T ELISA kits trial application
Product Type ELISA kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation SP
Target Name substance P (SP)
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %88-97
1:2Average %100
Range %96-103
1:4Average %91
Range %86-94
1:8Average %97
Range %92-100
Recovery
The recovery of bovine SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9487-99
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.526 2.432 2.479 2.342
51.948 1.979 1.964 1.827
2.51.279 1.223 1.251 1.114
1.250.802 0.785 0.794 0.657
0.6250.490 0.472 0.481 0.344
0.3120.342 0.337 0.340 0.203
0.1560.242 0.238 0.240 0.103
00.138 0.136 0.137
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Q&A and Customer Reviews

Q&A
Q:

Please email me information
How to maintain the plasma / serum until the test is done (test tube type, additional substances, freezer, etc.(

A:
Thanks for your interest in this item#CSB-EQ027765BO,Bovine substance P (SP) ELISA kit
Regarding the collection and storage of sample,pls kindly refer to the part "SAMPLE COLLECTION AND STORAGE" on Page 6 and Page 7 of the manual.As to other materials such as test tube type, additional substances, freezer, etc.,if no specific requirement,then ordinary types are ok.You can also just follow the manual about it.
If any questions,pls let me know.
Customer Reviews

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