Bovine substance P (SP) ELISA kit

Code CSB-EQ027765BO
See More Details
Product Type ELISA kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation SP
Target Name substance P (SP)
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %88-97
1:2Average %100
Range %96-103
1:4Average %91
Range %86-94
1:8Average %97
Range %92-100
Recovery
The recovery of bovine SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9487-99
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.526 2.432 2.479 2.342
51.948 1.979 1.964 1.827
2.51.279 1.223 1.251 1.114
1.250.802 0.785 0.794 0.657
0.6250.490 0.472 0.481 0.344
0.3120.342 0.337 0.340 0.203
0.1560.242 0.238 0.240 0.103
00.138 0.136 0.137
Still Have Questions? Leave a Message or Start an on-line Chat

Related Products

SP ELISA Kit

SP ELISA Kit for Homo sapiens (Human)

SP ELISA Kit for Rattus norvegicus (Rat)

SP ELISA Kit for Guinea Sus scrofa (Pig)

SP ELISA Kit for Mus musculus (Mouse)

SP ELISA Kit for Sus scrofa (Pig)

SP ELISA Kit for Oryctolagus cuniculus (Rabbit)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.