Bovine substance P (SP) ELISA kit

Code CSB-EQ027765BO
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Product Type ELISA kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation SP
Target Name substance P (SP)
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Neuroscience
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
1:1Average %94
Range %88-97
1:2Average %100
Range %96-103
1:4Average %91
Range %86-94
1:8Average %97
Range %92-100
The recovery of bovine SP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9487-99
EDTA plasma (n=4)9287-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
102.526 2.432 2.479 2.342
51.948 1.979 1.964 1.827
2.51.279 1.223 1.251 1.114
1.250.802 0.785 0.794 0.657
0.6250.490 0.472 0.481 0.344
0.3120.342 0.337 0.340 0.203
0.1560.242 0.238 0.240 0.103
00.138 0.136 0.137
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