Canine Beta-Endorphin,β-EP ELISA Kit

Code CSB-E06833c
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Lead Time 3-5 working days
Abbreviation β-EP
Target Name Beta-Endorphin,β-EP
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma
Detection Range 20 pg/mL- 4000 pg/mL
Sensitivity 10 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cancer
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Precision
Intra-assay Precision (Precision within an assay): CV%<15%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<15%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine β-EP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 96    
Range % 90-102    
1:2 Average % 93    
Range % 88-98    
1:4 Average % 95    
Range % 91-100    
1:8 Average % 95    
Range % 84-99    
Recovery
The recovery of canine β-EP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 97 87-102    
EDTA plasma (n=4) 96 90-103    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
               
               
               
               
               
               
               
               
               
               
               
               
               
pg/ml OD1 OD2 Average      
0 2.613 2.511 2.562      
20 1.425 1.389 1.407      
80 0.973 1.008 0.991      
320 0.495 0.504 0.500      
1000 0.342 0.334 0.338      
4000 0.150 0.153 0.152      
               
References
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