Canine Interleukin 17(IL-17)ELISA Kit

Code CSB-E17935c
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q687Y7
Lead Time 3-5 working days
Abbreviation IL17
Target Name Interleukin 17(IL-17)
Alias CTLA8, IL-17, IL-17A, IL17, cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|cytotoxic T-lymphocyte-associated serine esterase 8|interleukin 17 (cytotoxic T-ly
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
HGNC 5981
RGD 2888
MGI 107364
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 89
Range % 85-93
1:2 Average % 86
Range % 81-89
1:4 Average % 95
Range % 92-97
1:8 Average % 105
Range % 101-109
Recovery
The recovery of canine IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 88 83-90
EDTA plasma (n=4) 104 101-109
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
2000 2.809 2.936 2.873 2.778
1000 2.311 2.205 2.258 2.163
500 1.572 1.568 1.570 1.475
250 0.890 0.887 0.889 0.794
125 0.495 0.478 0.487 0.392
62.5 0.222 0.235 0.229 0.134
31.25 0.154 0.160 0.157 0.062
0 0.094 0.096 0.095  
References
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Function Ligand for IL17RA and IL17RC. The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC. Involved in inducing stromal cells to produce proinflammatory and hematopoietic cytokines.
Subcellular Location Secreted
Protein Families IL-17 family
Database Links

KEGG: bta:282863

STRING: 9913.ENSBTAP00000002786

UniGene: Bt.12772

Pathway IL-17 signaling pathway
Th17 cell differentiation

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